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5z96

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Structure of the mouse TRPC4 ion channel

5z96, resolution 3.28Å

Structural highlights

5z96 is a 4 chain structure with sequence from Lk3 transgenic mice. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Gene:	Trpc4, Trrp4 (LK3 transgenic mice)
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[TRPC4_MOUSE] Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Acts as a cell-cell contact-dependent endothelial calcium entry channel. Has also been shown to be calcium-selective (By similarity). May also be activated by intracellular calcium store depletion. Trpc4 deficient mice lack a store-operated calcium entry in endothelial cells.^[1]

Publication Abstract from PubMed

Members of the transient receptor potential (TRP) ion channels conduct cations into cells. They mediate functions ranging from neuronally mediated hot and cold sensation to intracellular organellar and primary ciliary signaling. Here we report a cryo-electron microscopy (cryo-EM) structure of TRPC4 in its unliganded (apo) state to an overall resolution of 3.3 A. The structure reveals a unique architecture with a long pore loop stabilized by a disulfide bond. Beyond the shared tetrameric six-transmembrane fold, the TRPC4 structure deviates from other TRP channels with a unique cytosolic domain. This unique cytosolic N-terminal domain forms extensive aromatic contacts with the TRP and the C-terminal domains. The comparison of our structure with other known TRP structures provides molecular insights into TRPC4 ion selectivity and extends our knowledge of the diversity and evolution of the TRP channels.

Structure of the mouse TRPC4 ion channel.,Duan J, Li J, Zeng B, Chen GL, Peng X, Zhang Y, Wang J, Clapham DE, Li Z, Zhang J Nat Commun. 2018 Aug 6;9(1):3102. doi: 10.1038/s41467-018-05247-9. PMID:30082700^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Freichel M, Suh SH, Pfeifer A, Schweig U, Trost C, Weissgerber P, Biel M, Philipp S, Freise D, Droogmans G, Hofmann F, Flockerzi V, Nilius B. Lack of an endothelial store-operated Ca2+ current impairs agonist-dependent vasorelaxation in TRP4-/- mice. Nat Cell Biol. 2001 Feb;3(2):121-7. PMID:11175743 doi:http://dx.doi.org/10.1038/35055019
↑ Duan J, Li J, Zeng B, Chen GL, Peng X, Zhang Y, Wang J, Clapham DE, Li Z, Zhang J. Structure of the mouse TRPC4 ion channel. Nat Commun. 2018 Aug 6;9(1):3102. doi: 10.1038/s41467-018-05247-9. PMID:30082700 doi:http://dx.doi.org/10.1038/s41467-018-05247-9

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5z96"

@@ Line 3: / Line 3: @@
 <SX load='5z96' size='340' side='right' viewer='molstar' caption='[[5z96]], [[Resolution|resolution]] 3.28&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5z96]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z96 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Z96 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5z96]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z96 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5Z96 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LPP:2-(HEXADECANOYLOXY)-1-[(PHOSPHONOOXY)METHYL]ETHYL+HEXADECANOATE'>LPP</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=Y01:CHOLESTEROL+HEMISUCCINATE'>Y01</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LPP:2-(HEXADECANOYLOXY)-1-[(PHOSPHONOOXY)METHYL]ETHYL+HEXADECANOATE'>LPP</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=Y01:CHOLESTEROL+HEMISUCCINATE'>Y01</scene></td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Trpc4, Trrp4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5z96 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z96 OCA], [http://pdbe.org/5z96 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5z96 RCSB], [http://www.ebi.ac.uk/pdbsum/5z96 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5z96 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5z96 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z96 OCA], [http://pdbe.org/5z96 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5z96 RCSB], [http://www.ebi.ac.uk/pdbsum/5z96 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5z96 ProSAT]</span></td></tr>
 </table>
 == Function ==

5z96

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Current revision

Structure of the mouse TRPC4 ion channel

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

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