6bm0

From Proteopedia

(Difference between revisions)

Revision as of 23:24, 10 April 2020

Cryo-EM structure of human CPSF-160-WDR33 complex at 3.8 A resolution

6bm0, resolution 3.80Å

Structural highlights

6bm0 is a 2 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	CPSF1, CPSF160 (HUMAN), WDR33, WDC146 (HUMAN)
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[CPSF1_HUMAN] Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. This subunit is involved in the RNA recognition step of the polyadenylation reaction.^[1] [WDR33_HUMAN] Essential for both cleavage and polyadenylation of pre-mRNA 3' ends.^[2]

Publication Abstract from PubMed

Nearly all eukaryotic messenger RNA precursors must undergo cleavage and polyadenylation at their 3'-end for maturation. A crucial step in this process is the recognition of the AAUAAA polyadenylation signal (PAS), and the molecular mechanism of this recognition has been a long-standing problem. Here, we report the cryo-electron microscopy structure of a quaternary complex of human CPSF-160, WDR33, CPSF-30, and an AAUAAA RNA at 3.4-A resolution. Strikingly, the AAUAAA PAS assumes an unusual conformation that allows this short motif to be bound directly by both CPSF-30 and WDR33. The A1 and A2 bases are recognized specifically by zinc finger 2 (ZF2) of CPSF-30 and the A4 and A5 bases by ZF3. Interestingly, the U3 and A6 bases form an intramolecular Hoogsteen base pair and directly contact WDR33. CPSF-160 functions as an essential scaffold and preorganizes CPSF-30 and WDR33 for high-affinity binding to AAUAAA. Our findings provide an elegant molecular explanation for how PAS sequences are recognized for mRNA 3'-end formation.

Molecular basis for the recognition of the human AAUAAA polyadenylation signal.,Sun Y, Zhang Y, Hamilton K, Manley JL, Shi Y, Walz T, Tong L Proc Natl Acad Sci U S A. 2017 Dec 5. pii: 1718723115. doi:, 10.1073/pnas.1718723115. PMID:29208711^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kaufmann I, Martin G, Friedlein A, Langen H, Keller W. Human Fip1 is a subunit of CPSF that binds to U-rich RNA elements and stimulates poly(A) polymerase. EMBO J. 2004 Feb 11;23(3):616-26. Epub 2004 Jan 29. PMID:14749727 doi:http://dx.doi.org/10.1038/sj.emboj.7600070
↑ Shi Y, Di Giammartino DC, Taylor D, Sarkeshik A, Rice WJ, Yates JR 3rd, Frank J, Manley JL. Molecular architecture of the human pre-mRNA 3' processing complex. Mol Cell. 2009 Feb 13;33(3):365-76. doi: 10.1016/j.molcel.2008.12.028. PMID:19217410 doi:http://dx.doi.org/10.1016/j.molcel.2008.12.028
↑ Sun Y, Zhang Y, Hamilton K, Manley JL, Shi Y, Walz T, Tong L. Molecular basis for the recognition of the human AAUAAA polyadenylation signal. Proc Natl Acad Sci U S A. 2017 Dec 5. pii: 1718723115. doi:, 10.1073/pnas.1718723115. PMID:29208711 doi:http://dx.doi.org/10.1073/pnas.1718723115

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6bm0"

@@ Line 3: / Line 3: @@
 <SX load='6bm0' size='340' side='right' viewer='molstar' caption='[[6bm0]], [[Resolution|resolution]] 3.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6bm0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BM0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BM0 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6bm0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BM0 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6BM0 FirstGlance]. <br>
 </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CPSF1, CPSF160 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), WDR33, WDC146 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bm0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bm0 OCA], [http://pdbe.org/6bm0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bm0 RCSB], [http://www.ebi.ac.uk/pdbsum/6bm0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bm0 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6bm0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bm0 OCA], [http://pdbe.org/6bm0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bm0 RCSB], [http://www.ebi.ac.uk/pdbsum/6bm0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bm0 ProSAT]</span></td></tr>
 </table>
 == Function ==

6bm0

From Proteopedia

Revision as of 23:24, 10 April 2020

Cryo-EM structure of human CPSF-160-WDR33 complex at 3.8 A resolution

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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