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6kz4

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Revision as of 02:33, 11 April 2020

YebT domain 5-7

6kz4, resolution 3.00Å

Structural highlights

6kz4 is a 6 chain structure with sequence from Ecoli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	yebT, b1834, JW1823 (ECOLI)
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[YEBT_ECOLI] Component of a transport pathway that contributes to membrane integrity (PubMed:27795327). May directly span the intermembrane space, facilitating the transport of substrates across the periplasm (Probable).^[1] ^[2]

Publication Abstract from PubMed

The outer membrane (OM) of Gram-negative bacteria is asymmetric, with lipopolysaccharides (LPSs) on the outer surface and phospholipids (PLs) on the inner surface. This unique organization of OM makes Gram-negative bacteria resistant to many toxic chemicals. How this asymmetric distribution of lipids is maintained has been studied for decades with previous reports of an Mla (Maintenance of OM Lipid Asymmetry) system to be involved. Furthermore, the OM of Gram-negative bacteria is about 20 nm away from inner membrane (IM) where the lipids are synthesized. Therefore, how nascent lipids travel across the periplasmic space and arrive at the inner surface of OM is another interesting question. YebT is a homologue of MlaD in the Mla pathway, but its role in lipid distribution of the OM and IM is largely unknown. Here we report the first high-resolution (~3.0 A) cryo-EM structure of full-length E. coli YebT in a substrate-bound state. Our structure with details of lipid interaction indicates that YebT is a lipid transporter spanning between IM and OM. We also demonstrate the symmetry mismatch in YebT and the existence of many other conformations of YebT revealing the intrinsic dynamics of this lipid channel. And a brief discussion on possible mechanisms of lipid transport is also included.

Cryo-EM Structure of a Bacterial Lipid Transporter YebT.,Liu C, Ma J, Wang J, Wang H, Zhang L J Mol Biol. 2020 Feb 14;432(4):1008-1019. doi: 10.1016/j.jmb.2019.12.008. Epub, 2019 Dec 21. PMID:31870848^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Nakayama T, Zhang-Akiyama QM. pqiABC and yebST, Putative mce Operons of Escherichia coli, Encode Transport Pathways and Contribute to Membrane Integrity. J Bacteriol. 2016 Dec 13;199(1). pii: JB.00606-16. doi: 10.1128/JB.00606-16., Print 2017 Jan 1. PMID:27795327 doi:http://dx.doi.org/10.1128/JB.00606-16
↑ Ekiert DC, Bhabha G, Isom GL, Greenan G, Ovchinnikov S, Henderson IR, Cox JS, Vale RD. Architectures of Lipid Transport Systems for the Bacterial Outer Membrane. Cell. 2017 Apr 6;169(2):273-285.e17. doi: 10.1016/j.cell.2017.03.019. PMID:28388411 doi:http://dx.doi.org/10.1016/j.cell.2017.03.019
↑ Liu C, Ma J, Wang J, Wang H, Zhang L. Cryo-EM Structure of a Bacterial Lipid Transporter YebT. J Mol Biol. 2020 Feb 14;432(4):1008-1019. doi: 10.1016/j.jmb.2019.12.008. Epub, 2019 Dec 21. PMID:31870848 doi:http://dx.doi.org/10.1016/j.jmb.2019.12.008

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6kz4"

@@ Line 3: / Line 3: @@
 <SX load='6kz4' size='340' side='right' viewer='molstar' caption='[[6kz4]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6kz4]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KZ4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6KZ4 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6kz4]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KZ4 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6KZ4 FirstGlance]. <br>
 </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">yebT, b1834, JW1823 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6kz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kz4 OCA], [http://pdbe.org/6kz4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kz4 RCSB], [http://www.ebi.ac.uk/pdbsum/6kz4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kz4 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6kz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kz4 OCA], [http://pdbe.org/6kz4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kz4 RCSB], [http://www.ebi.ac.uk/pdbsum/6kz4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kz4 ProSAT]</span></td></tr>
 </table>
 == Function ==

6kz4

From Proteopedia

Revision as of 02:33, 11 April 2020

YebT domain 5-7

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

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