6mgw

From Proteopedia

(Difference between revisions)

Revision as of 02:43, 11 April 2020

Structure of mechanically activated ion channel OSCA1.2 in LMNG

6mgw, resolution 3.50Å

Structural highlights

6mgw is a 2 chain structure with sequence from Arath. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	6mgv
Gene:	CSC1, At4g22120, F1N20.220 (ARATH)
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[CSC1_ARATH] Acts as an osmosensitive calcium-permeable cation channel. Specifically conducts cations including Ca(2+), K(+) and Na(+) in vitro. Inactivation or closure of the channel is calcium-dependent.^[1]

Publication Abstract from PubMed

Mechanically activated ion channels underlie touch, hearing, shear-stress sensing, and response to turgor pressure. OSCA/TMEM63s are a newly-identified family of eukaryotic mechanically activated ion channels opened by membrane tension. The structural underpinnings of OSCA/TMEM63 function are not explored. Here, we elucidate high resolution cryo-electron microscopy structures of OSCA1.2, revealing a dimeric architecture containing eleven transmembrane helices per subunit and surprising topological similarities to TMEM16 proteins. We locate the ion permeation pathway within each subunit by demonstrating that a conserved acidic residue is a determinant of channel conductance. Molecular dynamics simulations reveal membrane interactions, suggesting the role of lipids in OSCA1.2 gating. These results lay a foundation to decipher how the structural organization of OSCA/TMEM63 is suited for their roles as MA ion channels.

Cryo-EM structure of the mechanically activated ion channel OSCA1.2.,Jojoa Cruz S, Saotome K, Murthy SE, Tsui CCA, Sansom MS, Patapoutian A, Ward AB Elife. 2018 Nov 1;7. pii: 41845. doi: 10.7554/eLife.41845. PMID:30382939^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hou C, Tian W, Kleist T, He K, Garcia V, Bai F, Hao Y, Luan S, Li L. DUF221 proteins are a family of osmosensitive calcium-permeable cation channels conserved across eukaryotes. Cell Res. 2014 May;24(5):632-5. doi: 10.1038/cr.2014.14. Epub 2014 Feb 7. PMID:24503647 doi:http://dx.doi.org/10.1038/cr.2014.14
↑ Jojoa Cruz S, Saotome K, Murthy SE, Tsui CCA, Sansom MS, Patapoutian A, Ward AB. Cryo-EM structure of the mechanically activated ion channel OSCA1.2. Elife. 2018 Nov 1;7. pii: 41845. doi: 10.7554/eLife.41845. PMID:30382939 doi:http://dx.doi.org/10.7554/eLife.41845

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6mgw"

@@ Line 3: / Line 3: @@
 <SX load='6mgw' size='340' side='right' viewer='molstar' caption='[[6mgw]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6mgw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MGW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MGW FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6mgw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MGW OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6MGW FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6mgv|6mgv]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CSC1, At4g22120, F1N20.220 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mgw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mgw OCA], [http://pdbe.org/6mgw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mgw RCSB], [http://www.ebi.ac.uk/pdbsum/6mgw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mgw ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6mgw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mgw OCA], [http://pdbe.org/6mgw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mgw RCSB], [http://www.ebi.ac.uk/pdbsum/6mgw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mgw ProSAT]</span></td></tr>
 </table>
 == Function ==

6mgw

From Proteopedia

Revision as of 02:43, 11 April 2020

Structure of mechanically activated ion channel OSCA1.2 in LMNG

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

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