This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

6mwq

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 02:59, 11 April 2020

Single particle cryoEM structure of a DARPin-aldolase platform in complex with GFP

6mwq, resolution 3.00Å

Structural highlights

6mwq is a 8 chain structure with sequence from Aeqvi and Synthetic construct sequences. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	ALDOA (SYNTHETIC CONSTRUCT sequences), GFP (AEQVI)
Activity:	Fructose-bisphosphate aldolase, with EC number 4.1.2.13
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[GFP_AEQVI] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.

Publication Abstract from PubMed

Solving protein structures by single-particle cryoelectron microscopy (cryo-EM) has become a crucial tool in structural biology. While exciting progress is being made toward the visualization of small macromolecules, the median protein size in both eukaryotes and bacteria is still beyond the reach of cryo-EM. To overcome this problem, we implemented a platform strategy in which a small protein target was rigidly attached to a large, symmetric base via a selectable adapter. Of our seven designs, the best construct used a designed ankyrin repeat protein (DARPin) rigidly fused to tetrameric rabbit muscle aldolase through a helical linker. The DARPin retained its ability to bind its target: GFP. We solved the structure of this complex to 3.0 A resolution overall, with 5-8 A resolution in the GFP region. As flexibility in the DARPin position limited the overall resolution of the target, we describe strategies to rigidify this element.

Fusion of DARPin to Aldolase Enables Visualization of Small Protein by Cryo-EM.,Yao Q, Weaver SJ, Mock JY, Jensen GJ Structure. 2019 Apr 25. pii: S0969-2126(19)30125-X. doi:, 10.1016/j.str.2019.04.003. PMID:31080120^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Yao Q, Weaver SJ, Mock JY, Jensen GJ. Fusion of DARPin to Aldolase Enables Visualization of Small Protein by Cryo-EM. Structure. 2019 Apr 25. pii: S0969-2126(19)30125-X. doi:, 10.1016/j.str.2019.04.003. PMID:31080120 doi:http://dx.doi.org/10.1016/j.str.2019.04.003

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6mwq"

@@ Line 3: / Line 3: @@
 <SX load='6mwq' size='340' side='right' viewer='molstar' caption='[[6mwq]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6mwq]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeqvi Aeqvi] and [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MWQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6MWQ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6mwq]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeqvi Aeqvi] and [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MWQ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6MWQ FirstGlance]. <br>
 </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ALDOA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=32630 SYNTHETIC CONSTRUCT sequences]), GFP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6100 AEQVI])</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fructose-bisphosphate_aldolase Fructose-bisphosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.13 4.1.2.13] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6mwq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mwq OCA], [http://pdbe.org/6mwq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mwq RCSB], [http://www.ebi.ac.uk/pdbsum/6mwq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mwq ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6mwq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mwq OCA], [http://pdbe.org/6mwq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mwq RCSB], [http://www.ebi.ac.uk/pdbsum/6mwq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mwq ProSAT]</span></td></tr>
 </table>
 == Function ==

6mwq

From Proteopedia

Revision as of 02:59, 11 April 2020

Single particle cryoEM structure of a DARPin-aldolase platform in complex with GFP

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox