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6oua

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Revision as of 04:13, 11 April 2020

Cryo-EM structure of the yeast Ctf3 complex

6oua, resolution 4.18Å

Structural highlights

6oua is a 3 chain structure with sequence from Atcc 18824. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	6nuw
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[CENPI_YEAST] Component of the central kinetochore, which mediates the attachment of the centromere to the mitotic spindle by forming essential interactions between the microtubule-associated outer kinetochore proteins and the centromere-associated inner kinetochore proteins. Required for establishing bipolar spindle-microtubule attachments and proper chromosome segregation. Component of the kinetochore, a multiprotein complex that assembles on centromeric DNA and attaches chromosomes to spindle microtubules, mediating chromosome segregation and sister chromatid segregation during meiosis and mitosis. Component of the inner kinetochore constitutive centromere-associated network (CCAN), which serves as a structural platform for outer kinetochore assembly.^[1] [CENPK_YEAST] Component of the kinetochore, a multiprotein complex that assembles on centromeric DNA and attaches chromosomes to spindle microtubules, mediating chromosome segregation and sister chromatid segregation during meiosis and mitosis. Component of the inner kinetochore constitutive centromere-associated network (CCAN), which serves as a structural platform for outer kinetochore assembly.^[2] [CENPH_YEAST] Component of the kinetochore, a multiprotein complex that assembles on centromeric DNA and attaches chromosomes to spindle microtubules, mediating chromosome segregation and sister chromatid segregation during meiosis and mitosis. Component of the inner kinetochore constitutive centromere-associated network (CCAN), which serves as a structural platform for outer kinetochore assembly.^[3]

Publication Abstract from PubMed

Kinetochores are the chromosomal attachment points for spindle microtubules. They are also signaling hubs that control major cell cycle transitions and coordinate chromosome folding. Most well-studied eukaryotes rely on a conserved set of factors, which are divided among two loosely-defined groups, for these functions. Outer kinetochore proteins contact microtubules or regulate this contact directly. Inner kinetochore proteins designate the kinetochore assembly site by recognizing a specialized nucleosome containing the H3 variant Cse4/CENP-A. We previously determined the structure, resolved by cryo-electron microscopy (cryo-EM), of the yeast Ctf19 complex (Ctf19c, homologous to the vertebrate CCAN), providing a high-resolution view of inner kinetochore architecture (Hinshaw and Harrison, 2019). We now extend these observations by reporting a near-atomic model of the Ctf3 complex, the outermost Ctf19c sub-assembly seen in our original cryo-EM density. The model is sufficiently well-determined by the new data to enable molecular interpretation of Ctf3 recruitment and function.

The structure of the yeast Ctf3 complex.,Hinshaw SM, Dates AN, Harrison SC Elife. 2019 Jun 13;8. pii: 48215. doi: 10.7554/eLife.48215. PMID:31194673^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Cheeseman IM, Anderson S, Jwa M, Green EM, Kang J, Yates JR 3rd, Chan CS, Drubin DG, Barnes G. Phospho-regulation of kinetochore-microtubule attachments by the Aurora kinase Ipl1p. Cell. 2002 Oct 18;111(2):163-72. PMID:12408861
↑ Poddar A, Roy N, Sinha P. MCM21 and MCM22, two novel genes of the yeast Saccharomyces cerevisiae are required for chromosome transmission. Mol Microbiol. 1999 Jan;31(1):349-60. PMID:9987135
↑ Sanyal K, Ghosh SK, Sinha P. The MCM16 gene of the yeast Saccharomyces cerevisiae is required for chromosome segregation. Mol Gen Genet. 1998 Nov;260(2-3):242-50. PMID:9862478
↑ Hinshaw SM, Dates AN, Harrison SC. The structure of the yeast Ctf3 complex. Elife. 2019 Jun 13;8. pii: 48215. doi: 10.7554/eLife.48215. PMID:31194673 doi:http://dx.doi.org/10.7554/eLife.48215

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6oua"

@@ Line 3: / Line 3: @@
 <SX load='6oua' size='340' side='right' viewer='molstar' caption='[[6oua]], [[Resolution|resolution]] 4.18&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6oua]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OUA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6OUA FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6oua]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OUA OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6OUA FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6nuw|6nuw]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6oua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6oua OCA], [http://pdbe.org/6oua PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6oua RCSB], [http://www.ebi.ac.uk/pdbsum/6oua PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6oua ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6oua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6oua OCA], [http://pdbe.org/6oua PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6oua RCSB], [http://www.ebi.ac.uk/pdbsum/6oua PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6oua ProSAT]</span></td></tr>
 </table>
 == Function ==

6oua

From Proteopedia

Revision as of 04:13, 11 April 2020

Cryo-EM structure of the yeast Ctf3 complex

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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