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6qd1

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MloK1 model from single particle analysis of 2D crystals, class 5 (intermediate compact conformation)

6qd1, resolution 5.40Å

Structural highlights

6qd1 is a 4 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	6i9d, 6iax, 6qcy, 6qcz, 6qd0, 6qd2, 6qd3, 6qd4
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[CNGK1_RHILO] Cyclic nucleotide-regulated potassium channel activated by cAMP.^[1]

Publication Abstract from PubMed

Electron crystallography can reveal the structure of membrane proteins within 2D crystals under close-to-native conditions. High-resolution structural information can only be reached if crystals are perfectly flat and highly ordered. In practice, such crystals are difficult to obtain. Available image unbending algorithms correct for disorder, but only perform well on images of non-tilted, flat crystals, while out-of-plane distortions are not addressed. Here, we present an approach that employs single-particle refinement procedures to locally unbend crystals in 3D. With this method, density maps of the MloK1 potassium channel with a resolution of 4 A were obtained from images of 2D crystals that do not diffract beyond 10 A. Furthermore, 3D classification allowed multiple structures to be resolved, revealing a series of MloK1 conformations within a single 2D crystal. This conformational heterogeneity explains the poor diffraction observed and is related to channel function. The approach is implemented in the FOCUS package.

Retrieving high-resolution information from disordered 2D crystals by single-particle cryo-EM.,Righetto RD, Biyani N, Kowal J, Chami M, Stahlberg H Nat Commun. 2019 Apr 12;10(1):1722. doi: 10.1038/s41467-019-09661-5. PMID:30979902^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Clayton GM, Silverman WR, Heginbotham L, Morais-Cabral JH. Structural basis of ligand activation in a cyclic nucleotide regulated potassium channel. Cell. 2004 Nov 24;119(5):615-27. PMID:15550244 doi:10.1016/j.cell.2004.10.030
↑ Righetto RD, Biyani N, Kowal J, Chami M, Stahlberg H. Retrieving high-resolution information from disordered 2D crystals by single-particle cryo-EM. Nat Commun. 2019 Apr 12;10(1):1722. doi: 10.1038/s41467-019-09661-5. PMID:30979902 doi:http://dx.doi.org/10.1038/s41467-019-09661-5

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6qd1"

@@ Line 3: / Line 3: @@
 <SX load='6qd1' size='340' side='right' viewer='molstar' caption='[[6qd1]], [[Resolution|resolution]] 5.40&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6qd1]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QD1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QD1 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6qd1]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QD1 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6QD1 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6i9d|6i9d]], [[6iax|6iax]], [[6qcy|6qcy]], [[6qcz|6qcz]], [[6qd0|6qd0]], [[6qd2|6qd2]], [[6qd3|6qd3]], [[6qd4|6qd4]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qd1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qd1 OCA], [http://pdbe.org/6qd1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qd1 RCSB], [http://www.ebi.ac.uk/pdbsum/6qd1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qd1 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6qd1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qd1 OCA], [http://pdbe.org/6qd1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qd1 RCSB], [http://www.ebi.ac.uk/pdbsum/6qd1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qd1 ProSAT]</span></td></tr>
 </table>
 == Function ==

6qd1

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Current revision

MloK1 model from single particle analysis of 2D crystals, class 5 (intermediate compact conformation)

Structural highlights

Function

Publication Abstract from PubMed

References

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