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6sfw

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Cryo-EM Structure of the ClpX component of the ClpXP1/2 degradation machinery.

6sfw, resolution 6.00Å

Structural highlights

6sfw is a 6 chain structure with sequence from "bacterium_monocytogenes_hominis"_nyfeldt_1932 "bacterium monocytogenes hominis" nyfeldt 1932. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	6sfx
Gene:	clpX, ARJ20_08095, B4Y57_02215, D3B94_07125, DWE46_10580, DWE48_07885, FA029_07915, FORC68_1290, LmNIHS28_00678 ("Bacterium monocytogenes hominis" Nyfeldt 1932)
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[L8DZH5_LISMN] ATP-dependent specificity component of the Clp protease. It directs the protease to specific substrates. Can perform chaperone functions in the absence of ClpP.[HAMAP-Rule:MF_00175][SAAS:SAAS01076750]

Publication Abstract from PubMed

The ClpXP machinery is a two-component protease complex that performs targeted protein degradation in bacteria and mitochondria. The complex consists of the AAA+ chaperone ClpX and the peptidase ClpP. The hexameric ClpX utilizes the energy of ATP binding and hydrolysis to engage, unfold and translocate substrates into the catalytic chamber of tetradecameric ClpP, where they are degraded. Formation of the complex involves a symmetry mismatch, because hexameric AAA+ rings bind axially to the opposing stacked heptameric rings of the tetradecameric ClpP. Here we present the cryo-EM structure of ClpXP from Listeria monocytogenes. We unravel the heptamer-hexamer binding interface and provide novel insight into the ClpX-ClpP cross-talk and activation mechanism. Comparison with available crystal structures of ClpP and ClpX in different states allows us to understand important aspects of the complex mode of action of ClpXP and provides a structural framework for future pharmacological applications.

Cryo-EM structure of the ClpXP protein degradation machinery.,Gatsogiannis C, Balogh D, Merino F, Sieber SA, Raunser S Nat Struct Mol Biol. 2019 Oct;26(10):946-954. doi: 10.1038/s41594-019-0304-0., Epub 2019 Oct 3. PMID:31582852^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Gatsogiannis C, Balogh D, Merino F, Sieber SA, Raunser S. Cryo-EM structure of the ClpXP protein degradation machinery. Nat Struct Mol Biol. 2019 Oct;26(10):946-954. doi: 10.1038/s41594-019-0304-0., Epub 2019 Oct 3. PMID:31582852 doi:http://dx.doi.org/10.1038/s41594-019-0304-0

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6sfw"

@@ Line 3: / Line 3: @@
 <SX load='6sfw' size='340' side='right' viewer='molstar' caption='[[6sfw]], [[Resolution|resolution]] 6.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6sfw]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_monocytogenes_hominis"_nyfeldt_1932 "bacterium monocytogenes hominis" nyfeldt 1932]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SFW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6SFW FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6sfw]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_monocytogenes_hominis"_nyfeldt_1932 "bacterium monocytogenes hominis" nyfeldt 1932]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SFW OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6SFW FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6sfx|6sfx]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">clpX, ARJ20_08095, B4Y57_02215, D3B94_07125, DWE46_10580, DWE48_07885, FA029_07915, FORC68_1290, LmNIHS28_00678 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1639 "Bacterium monocytogenes hominis" Nyfeldt 1932])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6sfw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sfw OCA], [http://pdbe.org/6sfw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6sfw RCSB], [http://www.ebi.ac.uk/pdbsum/6sfw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6sfw ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6sfw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sfw OCA], [http://pdbe.org/6sfw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6sfw RCSB], [http://www.ebi.ac.uk/pdbsum/6sfw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6sfw ProSAT]</span></td></tr>
 </table>
 == Function ==

6sfw

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Current revision

Cryo-EM Structure of the ClpX component of the ClpXP1/2 degradation machinery.

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

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