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6v6c

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Revision as of 07:03, 11 April 2020

Structure of GCP6 in the native human gamma-tubulin ring complex

6v6c, resolution 4.50Å

Structural highlights

6v6c is a 1 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Disease

[GCP6_HUMAN] Autosomal recessive chorioretinopathy-microcephaly syndrome. The disease is caused by mutations affecting the gene represented in this entry.

Function

[GCP6_HUMAN] Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome.^[1]

Publication Abstract from PubMed

The gamma-tubulin ring complex (gamma-TuRC) is an essential regulator of centrosomal and acentrosomal microtubule formation, yet its structure is not known. Here, we present a cryo-EM reconstruction of the native human gamma-TuRC at approximately 3.8 A resolution, revealing an asymmetric, cone-shaped structure. Pseudo-atomic models indicate that GCP4, GCP5, and GCP6 form distinct Y-shaped assemblies that structurally mimic GCP2/GCP3 subcomplexes distal to the gamma-TuRC "seam." We also identify an unanticipated structural bridge that includes an actin-like protein and spans the gamma-TuRC lumen. Despite its asymmetric architecture, the gamma-TuRC arranges gamma-tubulins into a helical geometry poised to nucleate microtubules. Diversity in the gamma-TuRC subunits introduces large (>100,000 A(2)) surfaces in the complex that allow for interactions with different regulatory factors. The observed compositional complexity of the gamma-TuRC could self-regulate its assembly into a cone-shaped structure to control microtubule formation across diverse contexts, e.g., within biological condensates or alongside existing filaments.

Asymmetric Molecular Architecture of the Human gamma-Tubulin Ring Complex.,Wieczorek M, Urnavicius L, Ti SC, Molloy KR, Chait BT, Kapoor TM Cell. 2019 Dec 13. pii: S0092-8674(19)31369-8. doi: 10.1016/j.cell.2019.12.007. PMID:31862189^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Murphy SM, Preble AM, Patel UK, O'Connell KL, Dias DP, Moritz M, Agard D, Stults JT, Stearns T. GCP5 and GCP6: two new members of the human gamma-tubulin complex. Mol Biol Cell. 2001 Nov;12(11):3340-52. PMID:11694571
↑ Wieczorek M, Urnavicius L, Ti SC, Molloy KR, Chait BT, Kapoor TM. Asymmetric Molecular Architecture of the Human gamma-Tubulin Ring Complex. Cell. 2019 Dec 13. pii: S0092-8674(19)31369-8. doi: 10.1016/j.cell.2019.12.007. PMID:31862189 doi:http://dx.doi.org/10.1016/j.cell.2019.12.007

1 Structural highlights
2 Disease
3 Function
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6v6c"

@@ Line 3: / Line 3: @@
 <SX load='6v6c' size='340' side='right' viewer='molstar' caption='[[6v6c]], [[Resolution|resolution]] 4.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6v6c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6V6C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6V6C FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6v6c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6V6C OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6V6C FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6v6c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6v6c OCA], [http://pdbe.org/6v6c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6v6c RCSB], [http://www.ebi.ac.uk/pdbsum/6v6c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6v6c ProSAT]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6v6c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6v6c OCA], [http://pdbe.org/6v6c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6v6c RCSB], [http://www.ebi.ac.uk/pdbsum/6v6c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6v6c ProSAT]</span></td></tr>
 </table>
 == Disease ==
@@ Line 19: / Line 19: @@
 </div>
 <div class="pdbe-citations 6v6c" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Tubulin 3D Structures|Tubulin 3D Structures]]
 == References ==
 <references/>

6v6c

From Proteopedia

Revision as of 07:03, 11 April 2020

Structure of GCP6 in the native human gamma-tubulin ring complex

Structural highlights

Disease

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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