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6klt

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Revision as of 02:27, 11 April 2020

Troponin of cardiac thin filament in low-calcium state

6klt, resolution 12.00Å

Structural highlights

6klt is a 3 chain structure with sequence from Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[TNNC1_MOUSE] Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments. [TNNI3_MOUSE] Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. [TNNT2_MOUSE] Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

Publication Abstract from PubMed

Troponin is an essential component of striated muscle and it regulates the sliding of actomyosin system in a calcium-dependent manner. Despite its importance, the structure of troponin has been elusive due to its high structural heterogeneity. In this study, we analyzed the 3D structures of murine cardiac thin filaments using a cryo-electron microscope equipped with a Volta phase plate (VPP). Contrast enhancement by a VPP enabled us to reconstruct the entire repeat of the thin filament. We determined the orientation of troponin relative to F-actin and tropomyosin, and characterized the interactions between troponin and tropomyosin. This study provides a structural basis for understanding the molecular mechanism of actomyosin system.

Cryo-EM structures of cardiac thin filaments reveal the 3D architecture of troponin.,Oda T, Yanagisawa H, Wakabayashi T J Struct Biol. 2020 Jan 16:107450. doi: 10.1016/j.jsb.2020.107450. PMID:31954841^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Oda T, Yanagisawa H, Wakabayashi T. Cryo-EM structures of cardiac thin filaments reveal the 3D architecture of troponin. J Struct Biol. 2020 Jan 16:107450. doi: 10.1016/j.jsb.2020.107450. PMID:31954841 doi:http://dx.doi.org/10.1016/j.jsb.2020.107450

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6klt"

@@ Line 3: / Line 3: @@
 <SX load='6klt' size='340' side='right' viewer='molstar' caption='[[6klt]], [[Resolution|resolution]] 12.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6klt]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KLT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6KLT FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6klt]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KLT OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6KLT FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6klt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6klt OCA], [http://pdbe.org/6klt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6klt RCSB], [http://www.ebi.ac.uk/pdbsum/6klt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6klt ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6klt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6klt OCA], [http://pdbe.org/6klt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6klt RCSB], [http://www.ebi.ac.uk/pdbsum/6klt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6klt ProSAT]</span></td></tr>
 </table>
 == Function ==

6klt

From Proteopedia

Revision as of 02:27, 11 April 2020

Troponin of cardiac thin filament in low-calcium state

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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