6ru2

From Proteopedia

(Difference between revisions)

Current revision

Crystal Structure of Glucuronoyl Esterase from Cerrena unicolor

Structural highlights

6ru2 is a 2 chain structure with sequence from Cerrena unicolor. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.96Å
Ligands:	, , , ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GCE_CERUI Glucuronoyl esterase which may play a significant role in biomass degradation, as it is considered to disconnect hemicellulose from lignin through the hydrolysis of the ester bond between 4-O-methyl-D-glucuronic acid residues of glucuronoxylans and aromatic alcohols of lignin (PubMed:25425346, PubMed:26712478).^[1] ^[2]

Publication Abstract from PubMed

Structural and functional studies were conducted of the glucuronoyl esterase (GE) from Cerrena unicolor (CuGE), an enzyme catalyzing cleavage of lignin-carbohydrate ester bonds. CuGE is an alpha/beta-hydrolase belonging to carbohydrate esterase family 15 (CE15). The enzyme is modular, comprised of a catalytic and a carbohydrate-binding domain. SAXS data show CuGE as an elongated rigid molecule where the two domains are connected by a rigid linker. Detailed structural information of the catalytic domain in its apo- and inactivated form and complexes with aldouronic acids reveal well-defined binding of the 4-O-methyl-a-D-glucuronoyl moiety, not influenced by the nature of the attached xylo-oligosaccharide. Structural and sequence comparisons within CE15 enzymes reveal two distinct structural subgroups. CuGE belongs to the group of fungal CE15-B enzymes with an open and flat substrate-binding site. The interactions between CuGE and its natural substrates are explained and rationalized by the structural results, microscale thermophoresis and isothermal calorimetry.

The structural basis of fungal glucuronoyl esterase activity on natural substrates.,Ernst HA, Mosbech C, Langkilde AE, Westh P, Meyer AS, Agger JW, Larsen S Nat Commun. 2020 Feb 24;11(1):1026. doi: 10.1038/s41467-020-14833-9. PMID:32094331^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ d'Errico C, Jorgensen JO, Krogh KB, Spodsberg N, Madsen R, Monrad RN. Enzymatic degradation of lignin-carbohydrate complexes (LCCs): model studies using a fungal glucuronoyl esterase from Cerrena unicolor. Biotechnol Bioeng. 2015 May;112(5):914-22. doi: 10.1002/bit.25508. Epub 2015 Jan , 7. PMID:25425346 doi:http://dx.doi.org/10.1002/bit.25508
↑ d'Errico C, Borjesson J, Ding H, Krogh KB, Spodsberg N, Madsen R, Monrad RN. Improved biomass degradation using fungal glucuronoyl-esterases-hydrolysis of natural corn fiber substrate. J Biotechnol. 2016 Feb 10;219:117-23. doi: 10.1016/j.jbiotec.2015.12.024. Epub, 2015 Dec 19. PMID:26712478 doi:http://dx.doi.org/10.1016/j.jbiotec.2015.12.024
↑ Ernst HA, Mosbech C, Langkilde AE, Westh P, Meyer AS, Agger JW, Larsen S. The structural basis of fungal glucuronoyl esterase activity on natural substrates. Nat Commun. 2020 Feb 24;11(1):1026. doi: 10.1038/s41467-020-14833-9. PMID:32094331 doi:http://dx.doi.org/10.1038/s41467-020-14833-9

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6ru2"

@@ Line 3: / Line 3: @@
 <StructureSection load='6ru2' size='340' side='right'caption='[[6ru2]], [[Resolution|resolution]] 1.96&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6ru2]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RU2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6RU2 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6ru2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cerrena_unicolor Cerrena unicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RU2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6RU2 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.96&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6rtv|6rtv]], [[6ru1|6ru1]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ru2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ru2 OCA], [http://pdbe.org/6ru2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ru2 RCSB], [http://www.ebi.ac.uk/pdbsum/6ru2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ru2 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ru2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ru2 OCA], [https://pdbe.org/6ru2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ru2 RCSB], [https://www.ebi.ac.uk/pdbsum/6ru2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ru2 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/GCE_CERUI GCE_CERUI]] Glucuronoyl esterase which may play a significant role in biomass degradation, as it is considered to disconnect hemicellulose from lignin through the hydrolysis of the ester bond between 4-O-methyl-D-glucuronic acid residues of glucuronoxylans and aromatic alcohols of lignin (PubMed:25425346, PubMed:26712478).<ref>PMID:25425346</ref> <ref>PMID:26712478</ref>
+[https://www.uniprot.org/uniprot/GCE_CERUI GCE_CERUI] Glucuronoyl esterase which may play a significant role in biomass degradation, as it is considered to disconnect hemicellulose from lignin through the hydrolysis of the ester bond between 4-O-methyl-D-glucuronic acid residues of glucuronoxylans and aromatic alcohols of lignin (PubMed:25425346, PubMed:26712478).<ref>PMID:25425346</ref> <ref>PMID:26712478</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 19: / Line 19: @@
 </div>
 <div class="pdbe-citations 6ru2" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Methylesterase 3D structures|Methylesterase 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
+[[Category: Cerrena unicolor]]
 [[Category: Large Structures]]
-[[Category: Agger, J W]]
+[[Category: Agger JW]]
-[[Category: Ernst, H A]]
+[[Category: Ernst HA]]
-[[Category: Langkilde, A]]
+[[Category: Langkilde A]]
-[[Category: Larsen, S]]
+[[Category: Larsen S]]
-[[Category: Meyer, A]]
+[[Category: Meyer A]]
-[[Category: Mosbech, C]]
+[[Category: Mosbech C]]
-[[Category: Westh, P]]
+[[Category: Westh P]]
-[[Category: Alpha/beta-hydrolase]]
-[[Category: Ce15]]
-[[Category: Esterase]]
-[[Category: Hydrolase]]

6ru2

From Proteopedia

Current revision

Crystal Structure of Glucuronoyl Esterase from Cerrena unicolor

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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