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| | <StructureSection load='6k9y' size='340' side='right'caption='[[6k9y]], [[Resolution|resolution]] 2.20Å' scene=''> | | <StructureSection load='6k9y' size='340' side='right'caption='[[6k9y]], [[Resolution|resolution]] 2.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6k9y]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K9Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6K9Y FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6k9y]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K9Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6K9Y FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">VAT1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6k9y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k9y OCA], [http://pdbe.org/6k9y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6k9y RCSB], [http://www.ebi.ac.uk/pdbsum/6k9y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6k9y ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6k9y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k9y OCA], [https://pdbe.org/6k9y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6k9y RCSB], [https://www.ebi.ac.uk/pdbsum/6k9y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6k9y ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/VAT1_HUMAN VAT1_HUMAN]] Possesses ATPase activity (By similarity). Plays a part in calcium-regulated keratinocyte activation in epidermal repair mechanisms. Has no effect on cell proliferation. Negatively regulates mitochondrial fusion in cooperation with mitofusin proteins (MFN1-2).<ref>PMID:12898150</ref> <ref>PMID:17105775</ref> <ref>PMID:19508442</ref> | + | [https://www.uniprot.org/uniprot/VAT1_HUMAN VAT1_HUMAN] Possesses ATPase activity (By similarity). Plays a part in calcium-regulated keratinocyte activation in epidermal repair mechanisms. Has no effect on cell proliferation. Negatively regulates mitochondrial fusion in cooperation with mitofusin proteins (MFN1-2).<ref>PMID:12898150</ref> <ref>PMID:17105775</ref> <ref>PMID:19508442</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Endo, T]] | + | [[Category: Endo T]] |
| - | [[Category: Watanabe, Y]] | + | [[Category: Watanabe Y]] |
| - | [[Category: Oxidoreductase]]
| + | |
| - | [[Category: Phospholipid transfer protein]]
| + | |
| Structural highlights
Function
VAT1_HUMAN Possesses ATPase activity (By similarity). Plays a part in calcium-regulated keratinocyte activation in epidermal repair mechanisms. Has no effect on cell proliferation. Negatively regulates mitochondrial fusion in cooperation with mitofusin proteins (MFN1-2).[1] [2] [3]
Publication Abstract from PubMed
Eukaryotic cells are compartmentalized to form organelles, whose functions rely on proper phospholipid and protein transport. Here we determined the crystal structure of human VAT-1, a cytosolic soluble protein that was suggested to transfer phosphatidylserine, at 2.2 A resolution. We found that VAT-1 transferred not only phosphatidylserine but also other acidic phospholipids between membranes in vitro. Structure-based mutational analyses showed the presence of a possible lipid-binding cavity at the interface between the two sub-domains, and two tyrosine residues in the flexible loops facilitated phospholipid transfer, likely by functioning as a gate to this lipid-binding cavity. We also found that a basic and hydrophobic loop with two tryptophan residues was protruded from the molecule and facilitated binding to the acidic-lipid membranes, thereby achieving efficient phospholipid transfer.
Structural basis for inter-organelle phospholipid transport mediated by VAT-1.,Watanabe Y, Tamura Y, Kakuta C, Watanabe S, Endo T J Biol Chem. 2020 Jan 31. pii: RA119.011019. doi: 10.1074/jbc.RA119.011019. PMID:32005660[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Koch J, Foekens J, Timmermans M, Fink W, Wirzbach A, Kramer MD, Schaefer BM. Human VAT-1: a calcium-regulated activation marker of human epithelial cells. Arch Dermatol Res. 2003 Sep;295(5):203-10. doi: 10.1007/s00403-003-0421-8. Epub, 2003 Jul 30. PMID:12898150 doi:http://dx.doi.org/10.1007/s00403-003-0421-8
- ↑ Eura Y, Ishihara N, Oka T, Mihara K. Identification of a novel protein that regulates mitochondrial fusion by modulating mitofusin (Mfn) protein function. J Cell Sci. 2006 Dec 1;119(Pt 23):4913-25. doi: 10.1242/jcs.03253. Epub 2006 Nov , 14. PMID:17105775 doi:http://dx.doi.org/10.1242/jcs.03253
- ↑ Mertsch S, Becker M, Lichota A, Paulus W, Senner V. Vesicle amine transport protein-1 (VAT-1) is upregulated in glioblastomas and promotes migration. Neuropathol Appl Neurobiol. 2009 Aug;35(4):342-52. doi:, 10.1111/j.1365-2990.2009.00993.x. PMID:19508442 doi:http://dx.doi.org/10.1111/j.1365-2990.2009.00993.x
- ↑ Watanabe Y, Tamura Y, Kakuta C, Watanabe S, Endo T. Structural basis for inter-organelle phospholipid transport mediated by VAT-1. J Biol Chem. 2020 Jan 31. pii: RA119.011019. doi: 10.1074/jbc.RA119.011019. PMID:32005660 doi:http://dx.doi.org/10.1074/jbc.RA119.011019
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