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| | <StructureSection load='6l9s' size='340' side='right'caption='[[6l9s]], [[Resolution|resolution]] 2.00Å' scene=''> | | <StructureSection load='6l9s' size='340' side='right'caption='[[6l9s]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6l9s]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Roscs Roscs]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L9S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6L9S FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6l9s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Roseiflexus_castenholzii_DSM_13941 Roseiflexus castenholzii DSM 13941]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L9S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6L9S FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6kol|6kol]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6l9s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l9s OCA], [http://pdbe.org/6l9s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6l9s RCSB], [http://www.ebi.ac.uk/pdbsum/6l9s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6l9s ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6l9s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l9s OCA], [https://pdbe.org/6l9s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6l9s RCSB], [https://www.ebi.ac.uk/pdbsum/6l9s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6l9s ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A7NNM5_ROSCS A7NNM5_ROSCS] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Roscs]] | + | [[Category: Roseiflexus castenholzii DSM 13941]] |
| - | [[Category: Min, Z Z]] | + | [[Category: Min ZZ]] |
| - | [[Category: Wang, C]] | + | [[Category: Wang C]] |
| - | [[Category: Xu, X L]] | + | [[Category: Xu XL]] |
| - | [[Category: Zhang, C Y]] | + | [[Category: Zhang CY]] |
| - | [[Category: Auracyanin]]
| + | |
| - | [[Category: Blue copper protein]]
| + | |
| - | [[Category: Electron transfer]]
| + | |
| - | [[Category: Metal binding protein]]
| + | |
| - | [[Category: Na-dithionite reduced]]
| + | |
| - | [[Category: Roseiflexus castenholzii]]
| + | |
| Structural highlights
Function
A7NNM5_ROSCS
Publication Abstract from PubMed
Auracyanin (Ac) is a blue copper protein that mediates the electron transfer between Alternative Complex III (ACIII) and downstream electron acceptors in both fort chains of filamentous anoxygenic phototrophs. Here, we extracted and purified the air-oxidized RfxAc from the photoheterotrophically grown Roseiflexus castenholzii, and we illustrated the structural basis underlying its electron transferring features. Spectroscopic and enzymatic analyses demonstrated the reduction of air-oxidized RfxAc by the ACIII upon oxidation of menaquinol-4 and menaquinol-7. Crystal structures of the air-oxidized and Na-dithionite-reduced RfxAc at 2.2 and 2.0 A resolutions, respectively, showed that the copper ions are coordinated by His77, His146, Cys141, and Met151 in minor different geometries. The Cu(1)-S(delta) bond length increase of Met151, and the electron density Fourier differences at Cu(1) and His77 demonstrated their essential roles in the dithionite-induced reduction. Structural comparisons further revealed that the RfxAc contains a Chloroflexus aurantiacus Ac-A-like copper binding pocket and a hydrophobic patch surrounding the exposed edge of His146 imidazole, as well as an Ac-B-like Ser- and Thr-rich polar patch located at a different site on the surface. These spectroscopic and structural features allow RfxAc to mediate electron transfers between the ACIII and redox partners different from those of Ac-A and Ac-B. These results provide a structural basis for further investigating the electron transfer and energy transformation mechanism of bacterial photosynthesis, and the diversity and evolution of electron transport chains.
Structural basis underlying the electron transfer features of a blue copper protein auracyanin from the photosynthetic bacterium Roseiflexus castenholzii.,Wang C, Xin Y, Min Z, Qi J, Zhang C, Xu X Photosynth Res. 2020 Jan 13. pii: 10.1007/s11120-020-00709-y. doi:, 10.1007/s11120-020-00709-y. PMID:31933173[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Wang C, Xin Y, Min Z, Qi J, Zhang C, Xu X. Structural basis underlying the electron transfer features of a blue copper protein auracyanin from the photosynthetic bacterium Roseiflexus castenholzii. Photosynth Res. 2020 Jan 13. pii: 10.1007/s11120-020-00709-y. doi:, 10.1007/s11120-020-00709-y. PMID:31933173 doi:http://dx.doi.org/10.1007/s11120-020-00709-y
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