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| | <StructureSection load='5by8' size='340' side='right'caption='[[5by8]], [[Resolution|resolution]] 1.52Å' scene=''> | | <StructureSection load='5by8' size='340' side='right'caption='[[5by8]], [[Resolution|resolution]] 1.52Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5by8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspergillus_nidulans Aspergillus nidulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BY8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5BY8 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5by8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_nidulans_FGSC_A4 Aspergillus nidulans FGSC A4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BY8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5BY8 FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ANIA_10200 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=227321 Aspergillus nidulans]), AN3745.2, ANIA_03745 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=227321 Aspergillus nidulans])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5by8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5by8 OCA], [https://pdbe.org/5by8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5by8 RCSB], [https://www.ebi.ac.uk/pdbsum/5by8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5by8 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5by8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5by8 OCA], [http://pdbe.org/5by8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5by8 RCSB], [http://www.ebi.ac.uk/pdbsum/5by8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5by8 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/C8VMF9_EMENI C8VMF9_EMENI] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Aspergillus nidulans]] | + | [[Category: Aspergillus nidulans FGSC A4]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Calvino, F R]] | + | [[Category: Calvino FR]] |
| - | [[Category: Gumiero, A]] | + | [[Category: Gumiero A]] |
| - | [[Category: Kharde, S]] | + | [[Category: Kharde S]] |
| - | [[Category: Sinning, I]] | + | [[Category: Sinning I]] |
| - | [[Category: Wild, K]] | + | [[Category: Wild K]] |
| - | [[Category: 5s rnp]]
| + | |
| - | [[Category: Biosynthetic protein]]
| + | |
| - | [[Category: Brix domain]]
| + | |
| - | [[Category: Protein complex]]
| + | |
| - | [[Category: Protein-rna interaction]]
| + | |
| - | [[Category: Ribosome biogenesis]]
| + | |
| Structural highlights
Function
C8VMF9_EMENI
Publication Abstract from PubMed
The assembly of eukaryotic ribosomes is a hierarchical process involving about 200 biogenesis factors and a series of remodeling steps. The 5S RNP consisting of the 5S rRNA, RpL5 and RpL11 is recruited at an early stage, but has to rearrange during maturation of the pre-60S ribosomal subunit. Rpf2 and Rrs1 have been implicated in 5S RNP biogenesis, but their precise role was unclear. Here, we present the crystal structure of the Rpf2-Rrs1 complex from Aspergillus nidulans at 1.5 A resolution and describe it as Brix domain of Rpf2 completed by Rrs1 to form two anticodon-binding domains with functionally important tails. Fitting the X-ray structure into the cryo-EM density of a previously described pre-60S particle correlates with biochemical data. The heterodimer forms specific contacts with the 5S rRNA, RpL5 and the biogenesis factor Rsa4. The flexible protein tails of Rpf2-Rrs1 localize to the central protuberance. Two helices in the Rrs1 C-terminal tail occupy a strategic position to block the rotation of 25S rRNA and the 5S RNP. Our data provide a structural model for 5S RNP recruitment to the pre-60S particle and explain why removal of Rpf2-Rrs1 is necessary for rearrangements to drive 60S maturation.
The structure of Rpf2-Rrs1 explains its role in ribosome biogenesis.,Kharde S, Calvino FR, Gumiero A, Wild K, Sinning I Nucleic Acids Res. 2015 Jun 27. pii: gkv640. PMID:26117542[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kharde S, Calvino FR, Gumiero A, Wild K, Sinning I. The structure of Rpf2-Rrs1 explains its role in ribosome biogenesis. Nucleic Acids Res. 2015 Jun 27. pii: gkv640. PMID:26117542 doi:http://dx.doi.org/10.1093/nar/gkv640
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