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5c6k

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Revision as of 06:21, 7 June 2023

Bacteriophage P2 integrase catalytic domain

Structural highlights

5c6k is a 2 chain structure with sequence from Escherichia virus P2. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

VINT_BPP2 Integrase is necessary for integration of the phage into the host genome by site-specific recombination.

Publication Abstract from PubMed

Bacteriophage P2 is a temperate phage capable of integrating its DNA into the host genome by site-specific recombination upon lysogenization. Integration and excision of the phage genome requires P2 integrase, which performs recognition, cleavage and joining of DNA during these processes. This work presents the high-resolution crystal structure of the catalytic domain of P2 integrase, and analysis of the structure-function relationship of several previously identified non-functional P2 integrase mutants. The DNA binding area is characterized by a large positively charged patch, harboring key residues. The structure reveals potential for large dimer flexibility, likely essential for rearrangement of DNA strands upon integration and excision of the phage DNA.

Crystal structure of the bacteriophage P2 integrase catalytic domain.,Skaar K, Claesson M, Odegrip R, Hogbom M, Haggard-Ljungquist E, Stenmark P FEBS Lett. 2015 Oct 8. pii: S0014-5793(15)00875-3. doi:, 10.1016/j.febslet.2015.09.026. PMID:26453836^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Skaar K, Claesson M, Odegrip R, Hogbom M, Haggard-Ljungquist E, Stenmark P. Crystal structure of the bacteriophage P2 integrase catalytic domain. FEBS Lett. 2015 Oct 8. pii: S0014-5793(15)00875-3. doi:, 10.1016/j.febslet.2015.09.026. PMID:26453836 doi:http://dx.doi.org/10.1016/j.febslet.2015.09.026

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5c6k"

@@ Line 3: / Line 3: @@
 <StructureSection load='5c6k' size='340' side='right'caption='[[5c6k]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5c6k]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpp2 Bpp2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C6K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5C6K FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5c6k]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_P2 Escherichia virus P2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C6K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5C6K FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">int ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10679 BPP2])</td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5c6k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c6k OCA], [https://pdbe.org/5c6k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5c6k RCSB], [https://www.ebi.ac.uk/pdbsum/5c6k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5c6k ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5c6k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c6k OCA], [http://pdbe.org/5c6k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5c6k RCSB], [http://www.ebi.ac.uk/pdbsum/5c6k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5c6k ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/VINT_BPP2 VINT_BPP2]] Integrase is necessary for integration of the phage into the host genome by site-specific recombination.
+[https://www.uniprot.org/uniprot/VINT_BPP2 VINT_BPP2] Integrase is necessary for integration of the phage into the host genome by site-specific recombination.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 25: / Line 24: @@
 __TOC__
 </StructureSection>
-[[Category: Bpp2]]
+[[Category: Escherichia virus P2]]
 [[Category: Large Structures]]
-[[Category: Claesson, M]]
+[[Category: Claesson M]]
-[[Category: Eriksson, J]]
+[[Category: Eriksson J]]
-[[Category: Haggard-Ljungquist, E]]
+[[Category: Haggard-Ljungquist E]]
-[[Category: Hogbom, M]]
+[[Category: Hogbom M]]
-[[Category: Odegrip, R]]
+[[Category: Odegrip R]]
-[[Category: Skaar, K]]
+[[Category: Skaar K]]
-[[Category: Stenmark, P]]
+[[Category: Stenmark P]]
-[[Category: Hydrolase]]
-[[Category: Integrase]]
-[[Category: Integration]]
-[[Category: Site-specific recombination]]
-[[Category: Tyrosine recombinase]]

5c6k

From Proteopedia

Revision as of 06:21, 7 June 2023

Bacteriophage P2 integrase catalytic domain

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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