|
|
| Line 3: |
Line 3: |
| | <StructureSection load='5c8v' size='340' side='right'caption='[[5c8v]], [[Resolution|resolution]] 2.01Å' scene=''> | | <StructureSection load='5c8v' size='340' side='right'caption='[[5c8v]], [[Resolution|resolution]] 2.01Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5c8v]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Australian_sheep_blowfly Australian sheep blowfly]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C8V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5C8V FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5c8v]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lucilia_cuprina Lucilia cuprina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C8V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5C8V FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">LcaE7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7375 Australian sheep blowfly])</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5c8v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c8v OCA], [https://pdbe.org/5c8v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5c8v RCSB], [https://www.ebi.ac.uk/pdbsum/5c8v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5c8v ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5c8v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c8v OCA], [http://pdbe.org/5c8v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5c8v RCSB], [http://www.ebi.ac.uk/pdbsum/5c8v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5c8v ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q25252_LUCCU Q25252_LUCCU] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 23: |
Line 24: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Australian sheep blowfly]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Correy, G J]] | + | [[Category: Lucilia cuprina]] |
| - | [[Category: Jackson, C J]] | + | [[Category: Correy GJ]] |
| - | [[Category: Mabbitt, P D]] | + | [[Category: Jackson CJ]] |
| - | [[Category: Acetylcholinesterase]] | + | [[Category: Mabbitt PD]] |
| - | [[Category: Animal]]
| + | |
| - | [[Category: Australia]]
| + | |
| - | [[Category: Carboxylesterase]]
| + | |
| - | [[Category: Catalytic domain]]
| + | |
| - | [[Category: Diptera]]
| + | |
| - | [[Category: Drug resistance]]
| + | |
| - | [[Category: Gene]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Insect]]
| + | |
| - | [[Category: Insecticide]]
| + | |
| - | [[Category: Phosphorylation]]
| + | |
| - | [[Category: Protein structure]]
| + | |
| - | [[Category: Sheep]]
| + | |
| - | [[Category: Sheep disease]]
| + | |
| - | [[Category: Substrate specificity]]
| + | |
| Structural highlights
Function
Q25252_LUCCU
Publication Abstract from PubMed
The evolution of new enzymatic activity is rarely observed outside of the laboratory. In the agricultural pest Lucilia cuprina, a naturally occurring mutation (Gly137Asp) in alpha-esterase 7 (LcalphaE7) results in acquisition of organophosphate hydrolase activity and confers resistance to organophosphate insecticides. Here, we present an X-ray crystal structure of LcalphaE7:Gly137Asp that, along with kinetic data, suggests that Asp137 acts as a general base in the new catalytic mechanism. Unexpectedly, the conformation of Asp137 observed in the crystal structure obstructs the active site and is not catalytically productive. Molecular dynamics simulations reveal that alternative, catalytically competent conformers of Asp137 are sampled on the nanosecond time scale, although these states are less populated. Thus, although the mutation introduces the new reactive group responsible for organophosphate detoxification, the catalytic efficiency appears to be limited by conformational disorganization: the frequent sampling of low-energy nonproductive states. This result is consistent with a model of molecular evolution in which initial function-changing mutations can result in enzymes that display only a fraction of their catalytic potential due to conformational disorganization.
Conformational Disorganization within the Active Site of a Recently Evolved Organophosphate Hydrolase Limits Its Catalytic Efficiency.,Mabbitt PD, Correy GJ, Meirelles T, Fraser NJ, Coote ML, Jackson CJ Biochemistry. 2016 Mar 8;55(9):1408-17. doi: 10.1021/acs.biochem.5b01322. Epub, 2016 Feb 26. PMID:26881849[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Mabbitt PD, Correy GJ, Meirelles T, Fraser NJ, Coote ML, Jackson CJ. Conformational Disorganization within the Active Site of a Recently Evolved Organophosphate Hydrolase Limits Its Catalytic Efficiency. Biochemistry. 2016 Mar 8;55(9):1408-17. doi: 10.1021/acs.biochem.5b01322. Epub, 2016 Feb 26. PMID:26881849 doi:http://dx.doi.org/10.1021/acs.biochem.5b01322
|
