1av3

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{{STRUCTURE_1av3| PDB=1av3 | SCENE= }}
{{STRUCTURE_1av3| PDB=1av3 | SCENE= }}
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'''POTASSIUM CHANNEL BLOCKER KAPPA CONOTOXIN PVIIA FROM C. PURPURASCENS, NMR, 20 STRUCTURES'''
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===POTASSIUM CHANNEL BLOCKER KAPPA CONOTOXIN PVIIA FROM C. PURPURASCENS, NMR, 20 STRUCTURES===
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==Overview==
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BACKGROUND: kappa-PVIIA is a 27-residue polypeptide isolated from the venom of Conus purpurascens and is the first member of a new class of conotoxins that block potassium channels. By comparison to other ion channels of eukaryotic cell membranes, voltage-sensitive potassium channels are relatively simple and methodology has been developed for mapping their interactions with small-peptide toxins. PVIIA, therefore, is a valuable new probe of potassium channel structure. This study of the solution structure and mode of channel binding of PVIIA forms the basis for mapping the interacting residues at the conotoxin-ion channel interface. RESULTS: The three-dimensional structure of PVIIA resembles the triple-stranded beta sheet/cystine-knot motif formed by a number of toxic and inhibitory peptides. Subtle structural differences, predominantly in loops 2 and 4, are observed between PVIIA and other conotoxins with similar structural frameworks, however. Electrophysiological binding data suggest that PVIIA blocks channel currents by binding in a voltage-sensitive manner to the external vestibule and occluding the pore. Comparison of the electrostatic surface of PVIIA with that of the well-characterised potassium channel blocker charybdotoxin suggests a likely binding orientation for PVIIA. CONCLUSIONS: Although the structure of PVIIA is considerably different to that of the alphaK scorpion toxins, it has a similar mechanism of channel blockade. On the basis of a comparison of the structures of PVIIA and charybdotoxin, we suggest that Lys19 of PVIIA is the residue which is responsible for physically occluding the pore of the potassium channel.
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{{ABSTRACT_PUBMED_9438859}}
==About this Structure==
==About this Structure==
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1AV3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Conus_purpurascens Conus purpurascens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AV3 OCA].
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1AV3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Conus_purpurascens Conus purpurascens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AV3 OCA].
==Reference==
==Reference==
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[[Category: Kappa-conotoxin]]
[[Category: Kappa-conotoxin]]
[[Category: Potassium channel blocker]]
[[Category: Potassium channel blocker]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 17:39:01 2008''

Revision as of 14:39, 30 June 2008

Image:1av3.png

Template:STRUCTURE 1av3

POTASSIUM CHANNEL BLOCKER KAPPA CONOTOXIN PVIIA FROM C. PURPURASCENS, NMR, 20 STRUCTURES

Template:ABSTRACT PUBMED 9438859

About this Structure

1AV3 is a Single protein structure of sequence from Conus purpurascens. Full experimental information is available from OCA.

Reference

Solution structure and proposed binding mechanism of a novel potassium channel toxin kappa-conotoxin PVIIA., Scanlon MJ, Naranjo D, Thomas L, Alewood PF, Lewis RJ, Craik DJ, Structure. 1997 Dec 15;5(12):1585-97. PMID:9438859

Page seeded by OCA on Mon Jun 30 17:39:01 2008

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