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| | <StructureSection load='6y42' size='340' side='right'caption='[[6y42]], [[Resolution|resolution]] 4.30Å' scene=''> | | <StructureSection load='6y42' size='340' side='right'caption='[[6y42]], [[Resolution|resolution]] 4.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6y42]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Strvp Strvp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Y42 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6Y42 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6y42]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_venezuelae_ATCC_10712 Streptomyces venezuelae ATCC 10712]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Y42 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6Y42 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4.3Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SVEN_6563 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=953739 STRVP])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6y42 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6y42 OCA], [http://pdbe.org/6y42 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6y42 RCSB], [http://www.ebi.ac.uk/pdbsum/6y42 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6y42 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6y42 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6y42 OCA], [https://pdbe.org/6y42 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6y42 RCSB], [https://www.ebi.ac.uk/pdbsum/6y42 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6y42 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/F2RGC9_STRVP F2RGC9_STRVP] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Strvp]] | + | [[Category: Streptomyces venezuelae ATCC 10712]] |
| - | [[Category: Fontecilla-Camps, J C]] | + | [[Category: Fontecilla-Camps JC]] |
| - | [[Category: Volbeda, A]] | + | [[Category: Volbeda A]] |
| - | [[Category: Dna complex]]
| + | |
| - | [[Category: Iron sulfur cluster]]
| + | |
| - | [[Category: Redox sensor]]
| + | |
| - | [[Category: Transcription]]
| + | |
| Structural highlights
Function
F2RGC9_STRVP
Publication Abstract from PubMed
The [Fe2S2]-RsrR gene transcription regulator senses the redox status in bacteria by modulating DNA binding, while its cluster cycles between +1 and +2 states-only the latter binds DNA. We have previously shown that RsrR can undergo remarkable conformational changes involving a 100 degrees rotation of tryptophan 9 between exposed (Out) and buried (In) states. Here, we have used the chemical modification of Trp9, site-directed mutagenesis, and crystallographic and computational chemical studies to show that (i) the Out and In states correspond to oxidized and reduced RsrR, respectively, (ii) His33 is protonated in the In state due to a change in its pKa caused by cluster reduction, and (iii) Trp9 rotation is conditioned by the response of its dipole moment to environmental electrostatic changes. Our findings illustrate a novel function of protonation resulting from electron transfer.
Electron and Proton Transfers Modulate DNA Binding by the Transcription Regulator RsrR.,Crack JC, Amara P, Volbeda A, Mouesca JM, Rohac R, Pellicer Martinez MT, Huang CY, Gigarel O, Rinaldi C, Le Brun NE, Fontecilla-Camps JC J Am Chem Soc. 2020 Mar 3. doi: 10.1021/jacs.9b12250. PMID:32078310[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Crack JC, Amara P, Volbeda A, Mouesca JM, Rohac R, Pellicer Martinez MT, Huang CY, Gigarel O, Rinaldi C, Le Brun NE, Fontecilla-Camps JC. Electron and Proton Transfers Modulate DNA Binding by the Transcription Regulator RsrR. J Am Chem Soc. 2020 Mar 3. doi: 10.1021/jacs.9b12250. PMID:32078310 doi:http://dx.doi.org/10.1021/jacs.9b12250
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