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| <StructureSection load='5chh' size='340' side='right'caption='[[5chh]], [[Resolution|resolution]] 1.85Å' scene=''> | | <StructureSection load='5chh' size='340' side='right'caption='[[5chh]], [[Resolution|resolution]] 1.85Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
- | <table><tr><td colspan='2'>[[5chh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CHH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5CHH FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5chh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CHH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CHH FirstGlance]. <br> |
- | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
- | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">appY_1, ERS445055_02463, PA8380_27050, PAE221_02417, YQ19_15205 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5chh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5chh OCA], [https://pdbe.org/5chh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5chh RCSB], [https://www.ebi.ac.uk/pdbsum/5chh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5chh ProSAT]</span></td></tr> |
- | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5chh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5chh OCA], [http://pdbe.org/5chh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5chh RCSB], [http://www.ebi.ac.uk/pdbsum/5chh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5chh ProSAT]</span></td></tr> | + | |
| </table> | | </table> |
| + | == Function == |
| + | [https://www.uniprot.org/uniprot/Q9I0P9_PSEAE Q9I0P9_PSEAE] |
| <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| </div> | | </div> |
| <div class="pdbe-citations 5chh" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 5chh" style="background-color:#fffaf0;"></div> |
| + | |
| + | ==See Also== |
| + | *[[Transcriptional activator 3D structures|Transcriptional activator 3D structures]] |
| == References == | | == References == |
| <references/> | | <references/> |
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| </StructureSection> | | </StructureSection> |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
- | [[Category: Deng, X]] | + | [[Category: Pseudomonas aeruginosa]] |
- | [[Category: Gan, J H]] | + | [[Category: Deng X]] |
- | [[Category: Kang, H P]] | + | [[Category: Gan JH]] |
- | [[Category: Kong, W N]] | + | [[Category: Kang HP]] |
- | [[Category: Liang, H H]] | + | [[Category: Kong WN]] |
- | [[Category: Ma, J B]] | + | [[Category: Liang HH]] |
- | [[Category: Yu, X]] | + | [[Category: Ma JB]] |
- | [[Category: Zhao, J R]] | + | [[Category: Yu X]] |
- | [[Category: Zhu, M]] | + | [[Category: Zhao JR]] |
- | [[Category: Atp-dependent protease]]
| + | [[Category: Zhu M]] |
- | [[Category: Bacterial virulence]]
| + | |
- | [[Category: Protein degradation]]
| + | |
- | [[Category: Quorum-sensing]]
| + | |
- | [[Category: Transcription]]
| + | |
| Structural highlights
Function
Q9I0P9_PSEAE
Publication Abstract from PubMed
Although quorum-sensing (QS) systems are important regulators of virulence gene expression in the opportunistic human pathogen Pseudomonas aeruginosa, their detailed regulatory mechanisms have not been fully characterized. Here, we show that deletion of PA2588 resulted in increased production of pyocyanin and biofilm, as well as enhanced pathogenicity in a mouse model. To gain insights into the function of PA2588, we performed a ChIP-seq assay and identified 28 targets of PA2588, including the intergenic region between PA2588 and pqsH, which encodes the key synthase of Pseudomonas quinolone signal (PQS). Though the C-terminal domain was similar to DNA-binding regions of other AraC family members, structural studies revealed that PA2588 has a novel fold at the N-terminal region (NTR), and its C-terminal HTH (helix-turn-helix) domain is also unique in DNA recognition. We also demonstrated that the adaptor protein ClpS, an essential regulator of ATP-dependent protease ClpAP, directly interacted with PA2588 before delivering CdpR to ClpAP for degradation. We named PA2588 as CdpR (ClpAP-degradation and pathogenicity Regulator). Moreover, deletion of clpP or clpS/clpA promotes bacterial survival in a mouse model of acute pneumonia infection. Taken together, this study uncovered that CdpR is an important QS regulator, which can interact with the ClpAS-P system to regulate the expression of virulence factors and pathogenicity.
Structural and Molecular Mechanism of CdpR Involved in Quorum-Sensing and Bacterial Virulence in Pseudomonas aeruginosa.,Zhao J, Yu X, Zhu M, Kang H, Ma J, Wu M, Gan J, Deng X, Liang H PLoS Biol. 2016 Apr 27;14(4):e1002449. doi: 10.1371/journal.pbio.1002449., eCollection 2016 Apr. PMID:27119725[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Zhao J, Yu X, Zhu M, Kang H, Ma J, Wu M, Gan J, Deng X, Liang H. Structural and Molecular Mechanism of CdpR Involved in Quorum-Sensing and Bacterial Virulence in Pseudomonas aeruginosa. PLoS Biol. 2016 Apr 27;14(4):e1002449. doi: 10.1371/journal.pbio.1002449., eCollection 2016 Apr. PMID:27119725 doi:http://dx.doi.org/10.1371/journal.pbio.1002449
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