1ave

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{{STRUCTURE_1ave| PDB=1ave | SCENE= }}
{{STRUCTURE_1ave| PDB=1ave | SCENE= }}
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'''CRYSTAL STRUCTURE OF HEN EGG-WHITE APO-AVIDIN IN RELATION TO ITS THERMAL STABILITY PROPERTIES'''
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===CRYSTAL STRUCTURE OF HEN EGG-WHITE APO-AVIDIN IN RELATION TO ITS THERMAL STABILITY PROPERTIES===
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==Overview==
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The three-dimensional structure of hen egg-white apo-avidin, crystallized in a tetragonal crystal form, has been refined to a crystallographic R-factor of 0.164 (for the 6390 observed reflections in the 10.0 to 2.8 A resolution range). As in the case of holo-avidin, from which starting atomic co-ordinates were derived, the functional tetramer shows 2-pseudo 22 molecular symmetry. Each promoter is organized in an eight-stranded antiparallel orthogonal beta-barrel, with extended loop regions, which define the biotin binding pocket in the protomer core. In the absence of biotin the binding site is only partly occupied by water molecules. The structure of the binding site residues, as observed in apo-avidin, is highly complementary to that of the incoming biotin molecule, accounting for prompt and specific recognition. A crystal lattice contact may play a role in stabilizing the conformation of one protein loop, part of the biotin-binding pocket.
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(as it appears on PubMed at http://www.pubmed.gov), where 8289264 is the PubMed ID number.
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{{ABSTRACT_PUBMED_8289264}}
==About this Structure==
==About this Structure==
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[[Category: Pugliese, L.]]
[[Category: Pugliese, L.]]
[[Category: Biotin-binding protein]]
[[Category: Biotin-binding protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:44:22 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 17:40:23 2008''

Revision as of 14:40, 30 June 2008

Template:STRUCTURE 1ave

CRYSTAL STRUCTURE OF HEN EGG-WHITE APO-AVIDIN IN RELATION TO ITS THERMAL STABILITY PROPERTIES

Template:ABSTRACT PUBMED 8289264

About this Structure

1AVE is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.

Reference

Crystal structure of apo-avidin from hen egg-white., Pugliese L, Malcovati M, Coda A, Bolognesi M, J Mol Biol. 1994 Jan 7;235(1):42-6. PMID:8289264

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