1aw2

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{{STRUCTURE_1aw2| PDB=1aw2 | SCENE= }}
{{STRUCTURE_1aw2| PDB=1aw2 | SCENE= }}
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'''TRIOSEPHOSPHATE ISOMERASE OF VIBRIO MARINUS'''
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===TRIOSEPHOSPHATE ISOMERASE OF VIBRIO MARINUS===
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==Overview==
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The purification and characterization of triose-phosphate isomerase from the psychrophilic bacterium Vibrio marinus (vTIM) is described. Crystal structures of the vTIM-sulfate complex and the vTIM-2-phosphoglycolate complex (at a 2.7-A resolution) are also presented. The optimal growth temperature of Vibrio marinus is 15 degrees C. Stability studies show that vTIM is an unstable protein with a half-life of only 10 min at 25 degrees C. The vTIM sequence is most closely related to the sequence of Escherichia coli TIM (eTIM) (66% identity), and several unique structural features described for eTIM are also seen in vTIM, but eTIM is considerably more stable. The Td values of vTIM and eTIM, determined by calorimetric studies, are 41 and 54 degrees C, respectively. Amino acid sequence comparison reveals that vTIM has an alanine in loop 8 (at position 238), whereas all other TIM sequences known to date have a serine. The vTIM mutant A238S was produced and characterized. Compared with wild type, the catalytic efficiency of the A238S mutant is somewhat reduced, and its stability is considerably increased.
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(as it appears on PubMed at http://www.pubmed.gov), where 9442062 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9442062}}
==About this Structure==
==About this Structure==
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[[Category: Psychrophilic]]
[[Category: Psychrophilic]]
[[Category: Vibrio marinus]]
[[Category: Vibrio marinus]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 17:43:25 2008''

Revision as of 14:43, 30 June 2008

Image:1aw2.png

Template:STRUCTURE 1aw2

TRIOSEPHOSPHATE ISOMERASE OF VIBRIO MARINUS

Template:ABSTRACT PUBMED 9442062

About this Structure

1AW2 is a Single protein structure of sequence from Moritella marina. Full crystallographic information is available from OCA.

Reference

Triose-phosphate isomerase (TIM) of the psychrophilic bacterium Vibrio marinus. Kinetic and structural properties., Alvarez M, Zeelen JP, Mainfroid V, Rentier-Delrue F, Martial JA, Wyns L, Wierenga RK, Maes D, J Biol Chem. 1998 Jan 23;273(4):2199-206. PMID:9442062

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