6jq9
From Proteopedia
(Difference between revisions)
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<StructureSection load='6jq9' size='340' side='right'caption='[[6jq9]], [[Resolution|resolution]] 1.81Å' scene=''> | <StructureSection load='6jq9' size='340' side='right'caption='[[6jq9]], [[Resolution|resolution]] 1.81Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6jq9]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JQ9 OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[6jq9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Altsl Altsl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JQ9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6JQ9 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">LOR_107 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1537994 ALTSL])</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6jq9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jq9 OCA], [https://pdbe.org/6jq9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6jq9 RCSB], [https://www.ebi.ac.uk/pdbsum/6jq9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6jq9 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Marine macroalgae have gained considerable attention as renewable biomass sources. Ulvan is a water-soluble anionic polysaccharide, and its depolymerization into fermentable monosaccharides has great potential for the production of bioethanol or high-value food additives. Ulvan lyase from Alteromonas sp. (AsPL) utilizes a beta-elimination mechanism to cleave the glycosidic bond between rhamnose 3-sulfate and glucuronic acid, forming an unsaturated uronic acid at the non-reducing end. AsPL was active in the temperature range of 30-50 degrees C and pH values ranging from 7.5 to 9.5. Furthermore, AsPL was found to be halophilic, showing high activity and stability in the presence of up to 2.5M NaCl. The apparent Km and kcat values of AsPL are 3.19+/-0.37mgmL(-1) and 4.19+/-0.21s(-1), respectively. Crystal structure analysis revealed that AsPL adopts a beta-propeller fold with four anti-parallel beta-strands in each of the seven propeller blades. The acid residues at the protein surface and two Ca(2+) coordination sites contribute to its salt tolerance. The research on ulvan lyase has potential commercial value in the utilization of algal resources for biofuel production to relieve the environmental burden of petrochemicals. | ||
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| + | Biochemical characterization and structural analysis of ulvan lyase from marine Alteromonas sp. reveals the basis for its salt tolerance.,Qin HM, Gao D, Zhu M, Li C, Zhu Z, Wang H, Liu W, Tanokura M, Lu F Int J Biol Macromol. 2020 Mar 15;147:1309-1317. doi:, 10.1016/j.ijbiomac.2019.10.095. Epub 2019 Nov 18. PMID:31751708<ref>PMID:31751708</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6jq9" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Altsl]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Guo, Q Q]] | [[Category: Guo, Q Q]] | ||
[[Category: Qin, H M]] | [[Category: Qin, H M]] | ||
[[Category: Lyase]] | [[Category: Lyase]] | ||
Revision as of 20:11, 20 October 2021
Crystal structure of a lyase from Alteromonas sp.
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Categories: Altsl | Large Structures | Guo, Q Q | Qin, H M | Lyase
