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6iet

From Proteopedia

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Current revision

The crystal structure of TRIM66 PHD-Bromo domain

Structural highlights

6iet is a 1 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.101Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

TRI66_HUMAN May function as transcription repressor; The repressive effects are mediated, at least in part, by recruitment of deacetylase activity. May play a role as negative regulator of postmeiotic genes acting through CBX3 complex formation and centromere association (By similarity).

Publication Abstract from PubMed

Recognition of specific chromatin modifications by distinct structural domains within "reader" proteins plays a critical role in the maintenance of genomic stability. However, the specific mechanisms involved in this process remain unclear. Here we report that the PHD-Bromo tandem domain of tripartite motif-containing 66 (TRIM66) recognizes the unmodified H3R2-H3K4 and acetylated H3K56. The aberrant deletion of Trim66 results in severe DNA damage and genomic instability in embryonic stem cells (ESCs). Moreover, we find that the recognition of histone modification by TRIM66 is critical for DNA damage repair (DDR) in ESCs. TRIM66 recruits Sirt6 to deacetylate H3K56ac, negatively regulating the level of H3K56ac and facilitating the initiation of DDR. Importantly, Trim66-deficient blastocysts also exhibit higher levels of H3K56ac and DNA damage. Collectively, the present findings indicate the vital role of TRIM66 in DDR in ESCs, establishing the relationship between histone readers and maintenance of genomic stability.

TRIM66 reads unmodified H3R2K4 and H3K56ac to respond to DNA damage in embryonic stem cells.,Chen J, Wang Z, Guo X, Li F, Wei Q, Chen X, Gong D, Xu Y, Chen W, Liu Y, Kang J, Shi Y Nat Commun. 2019 Sep 19;10(1):4273. doi: 10.1038/s41467-019-12126-4. PMID:31537782^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Chen J, Wang Z, Guo X, Li F, Wei Q, Chen X, Gong D, Xu Y, Chen W, Liu Y, Kang J, Shi Y. TRIM66 reads unmodified H3R2K4 and H3K56ac to respond to DNA damage in embryonic stem cells. Nat Commun. 2019 Sep 19;10(1):4273. doi: 10.1038/s41467-019-12126-4. PMID:31537782 doi:http://dx.doi.org/10.1038/s41467-019-12126-4

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6iet"

Categories: Homo sapiens | Large Structures | Chen J

@@ Line 3: / Line 3: @@
 <StructureSection load='6iet' size='340' side='right'caption='[[6iet]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6iet]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IET OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IET FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6iet]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IET OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6IET FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.101&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TRIM66 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6iet FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6iet OCA], [http://pdbe.org/6iet PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6iet RCSB], [http://www.ebi.ac.uk/pdbsum/6iet PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6iet ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6iet FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6iet OCA], [https://pdbe.org/6iet PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6iet RCSB], [https://www.ebi.ac.uk/pdbsum/6iet PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6iet ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/TRI66_HUMAN TRI66_HUMAN]] May function as transcription repressor; The repressive effects are mediated, at least in part, by recruitment of deacetylase activity. May play a role as negative regulator of postmeiotic genes acting through CBX3 complex formation and centromere association (By similarity).
+[https://www.uniprot.org/uniprot/TRI66_HUMAN TRI66_HUMAN] May function as transcription repressor; The repressive effects are mediated, at least in part, by recruitment of deacetylase activity. May play a role as negative regulator of postmeiotic genes acting through CBX3 complex formation and centromere association (By similarity).
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
 [[Category: Large Structures]]
-[[Category: Chen, J]]
+[[Category: Chen J]]
-[[Category: Phd-bromo]]
-[[Category: Transcription]]
-[[Category: Trim66]]

6iet

From Proteopedia

Current revision

The crystal structure of TRIM66 PHD-Bromo domain

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

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