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1awz

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[[Image:1awz.jpg|left|200px]]
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{{STRUCTURE_1awz| PDB=1awz | SCENE= }}
{{STRUCTURE_1awz| PDB=1awz | SCENE= }}
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'''3D SOLUTION STRUCTURE OF HUMAN ANGIOGENIN DETERMINED BY 1H, 15N NMR SPECTROSCOPY, 30 STRUCTURES'''
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===3D SOLUTION STRUCTURE OF HUMAN ANGIOGENIN DETERMINED BY 1H, 15N NMR SPECTROSCOPY, 30 STRUCTURES===
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==Overview==
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Human angiogenin is a member of the pancreatic ribonuclease superfamily that induces blood vessel formation. Its three-dimensional solution structure has been determined to high resolution by heteronuclear NMR spectroscopy. 30 structures were calculated, based on a total of 1441 assigned NOE correlations, 64 coupling constants and 50 hydrogen bonds. The backbone atomic rms difference from the mean coordinates is 0.067 +/- 0.012 nm and 0.13 nm from the previously determined crystal structure. The side-chain of Gln117 was found to obstruct the active site as observed in the crystal state. There was no evidence of an alternative open form of angiogenin, although two sets of chemical shifts were observed for some residues, mainly around the active site and in the C-terminal segment. The topology of the ribonucleolytic active site is described with a particular emphasis on the conformation and protonation of active-site His residues. The side-chain of His114 adopts two main conformations in solution. In contrast to pancreatic ribonuclease A, His13 was shown to be more basic than His114, with pKa values of 6.65 and 6.05 respectively. The His47 residue is located in an environment very resistant to protonation with a pKa lower than 4.
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The line below this paragraph, {{ABSTRACT_PUBMED_9461294}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 9461294 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9461294}}
==About this Structure==
==About this Structure==
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1AWZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AWZ OCA].
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1AWZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AWZ OCA].
==Reference==
==Reference==
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[[Category: Endoribonuclease]]
[[Category: Endoribonuclease]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:47:33 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 17:46:14 2008''

Revision as of 14:46, 30 June 2008

Image:1awz.png

Template:STRUCTURE 1awz

3D SOLUTION STRUCTURE OF HUMAN ANGIOGENIN DETERMINED BY 1H, 15N NMR SPECTROSCOPY, 30 STRUCTURES

Template:ABSTRACT PUBMED 9461294

About this Structure

1AWZ is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

Three-dimensional solution structure of human angiogenin determined by 1H,15N-NMR spectroscopy--characterization of histidine protonation states and pKa values., Lequin O, Thuring H, Robin M, Lallemand JY, Eur J Biochem. 1997 Dec 15;250(3):712-26. PMID:9461294

Page seeded by OCA on Mon Jun 30 17:46:14 2008

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