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1axk

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{{STRUCTURE_1axk| PDB=1axk | SCENE= }}
{{STRUCTURE_1axk| PDB=1axk | SCENE= }}
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'''ENGINEERED BACILLUS BIFUNCTIONAL ENZYME GLUXYN-1'''
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===ENGINEERED BACILLUS BIFUNCTIONAL ENZYME GLUXYN-1===
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==Overview==
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The 1,3-1,4-beta-glucanase from Bacillus macerans (wtGLU) and the 1, 4-beta-xylanase from Bacillus subtilis (wtXYN) are both single-domain jellyroll proteins catalyzing similar enzymatic reactions. In the fusion protein GluXyn-1, the two proteins are joined by insertion of the entire XYN domain into a surface loop of cpMAC-57, a circularly permuted variant of wtGLU. GluXyn-1 was generated by protein engineering methods, produced in Escherichia coli and shown to fold spontaneously and have both enzymatic activities at wild-type level. The crystal structure of GluXyn-1 was determined at 2.1 A resolution and refined to R = 17.7% and R(free) = 22.4%. It shows nearly ideal, native-like folding of both protein domains and a small, but significant hinge bending between the domains. The active sites are independent and accessible explaining the observed enzymatic activity. Because in GluXyn-1 the complete XYN domain is inserted into the compact folding unit of GLU, the wild-type-like activity and tertiary structure of the latter proves that the folding process of GLU does not depend on intramolecular interactions that are short-ranged in the sequence. Insertion fusions of the GluXyn-1 type may prove to be an easy route toward more stable bifunctional proteins in which the two parts are more closely associated than in linear end-to-end protein fusions.
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{{ABSTRACT_PUBMED_9618460}}
==About this Structure==
==About this Structure==
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[[Category: Gluxyn-1]]
[[Category: Gluxyn-1]]
[[Category: Hybrid enzyme]]
[[Category: Hybrid enzyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:48:48 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 17:48:37 2008''

Revision as of 14:48, 30 June 2008

Image:1axk.png

Template:STRUCTURE 1axk

ENGINEERED BACILLUS BIFUNCTIONAL ENZYME GLUXYN-1

Template:ABSTRACT PUBMED 9618460

About this Structure

1AXK is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

Reference

Structure and function of the Bacillus hybrid enzyme GluXyn-1: native-like jellyroll fold preserved after insertion of autonomous globular domain., Ay J, Gotz F, Borriss R, Heinemann U, Proc Natl Acad Sci U S A. 1998 Jun 9;95(12):6613-8. PMID:9618460

Page seeded by OCA on Mon Jun 30 17:48:37 2008

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