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2noq

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Revision as of 10:35, 12 January 2022

Structure of ribosome-bound cricket paralysis virus IRES RNA

2noq, resolution 7.30Å

Structural highlights

2noq is a 8 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[RL5_YEAST] Binds 5S RNA and is required for 60S subunit assembly. [RL11B_YEAST] Binds to 5S ribosomal RNA.

Evolutionary Conservation

Checkto colour the structure by Evolutionary Conservation, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Internal ribosome entry sites (IRESs) facilitate an alternative, end-independent pathway of translation initiation. A particular family of dicistroviral IRESs can assemble elongation-competent 80S ribosomal complexes in the absence of canonical initiation factors and initiator transfer RNA. We present here a cryo-EM reconstruction of a dicistroviral IRES bound to the 80S ribosome. The resolution of the cryo-EM reconstruction, in the subnanometer range, allowed the molecular structure of the complete IRES in its active, ribosome-bound state to be solved. The structure, harboring three pseudoknot-containing domains, each with a specific functional role, shows how defined elements of the IRES emerge from a compactly folded core and interact with the key ribosomal components that form the A, P and E sites, where tRNAs normally bind. Our results exemplify the molecular strategy for recruitment of an IRES and reveal the dynamic features necessary for internal initiation.

Structure of the ribosome-bound cricket paralysis virus IRES RNA.,Schuler M, Connell SR, Lescoute A, Giesebrecht J, Dabrowski M, Schroeer B, Mielke T, Penczek PA, Westhof E, Spahn CM Nat Struct Mol Biol. 2006 Dec;13(12):1092-6. Epub 2006 Nov 19. PMID:17115051^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Schuler M, Connell SR, Lescoute A, Giesebrecht J, Dabrowski M, Schroeer B, Mielke T, Penczek PA, Westhof E, Spahn CM. Structure of the ribosome-bound cricket paralysis virus IRES RNA. Nat Struct Mol Biol. 2006 Dec;13(12):1092-6. Epub 2006 Nov 19. PMID:17115051 doi:10.1038/nsmb1177

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2noq"

@@ Line 3: / Line 3: @@
 <SX load='2noq' size='340' side='right' viewer='molstar' caption='[[2noq]], [[Resolution|resolution]] 7.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2noq]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NOQ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2NOQ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2noq]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NOQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NOQ FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2noq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2noq OCA], [http://pdbe.org/2noq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2noq RCSB], [http://www.ebi.ac.uk/pdbsum/2noq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2noq ProSAT]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2noq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2noq OCA], [https://pdbe.org/2noq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2noq RCSB], [https://www.ebi.ac.uk/pdbsum/2noq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2noq ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/RL5_YEAST RL5_YEAST]] Binds 5S RNA and is required for 60S subunit assembly. [[http://www.uniprot.org/uniprot/RL11B_YEAST RL11B_YEAST]] Binds to 5S ribosomal RNA.
+[[https://www.uniprot.org/uniprot/RL5_YEAST RL5_YEAST]] Binds 5S RNA and is required for 60S subunit assembly. [[https://www.uniprot.org/uniprot/RL11B_YEAST RL11B_YEAST]] Binds to 5S ribosomal RNA.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]

2noq

From Proteopedia

Revision as of 10:35, 12 January 2022

Structure of ribosome-bound cricket paralysis virus IRES RNA

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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