1azf

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[[Image:1azf.gif|left|200px]]
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{{Seed}}
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[[Image:1azf.png|left|200px]]
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{{STRUCTURE_1azf| PDB=1azf | SCENE= }}
{{STRUCTURE_1azf| PDB=1azf | SCENE= }}
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'''CHICKEN EGG WHITE LYSOZYME CRYSTAL GROWN IN BROMIDE SOLUTION'''
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===CHICKEN EGG WHITE LYSOZYME CRYSTAL GROWN IN BROMIDE SOLUTION===
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==Overview==
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Anions have been shown to play a dominant role in the crystallization of chicken egg-white lysozyme from salt solutions. Previous studies employing X-ray crystallography have found one chloride ion binding site in the tetragonal crystal form of the protein and four nitrate ion binding sites in the monoclinic form. In this study the anion positions in the tetragonal form were determined from the difference Fourier map obtained from lysozyme crystals grown in bromide and chloride solutions. Five possible anion-binding sites were found in this manner. Some of these sites were in pockets containing basic residues while others were near neutral, but polar, residues. The sole chloride ion binding site found in previous studies was confirmed, while four further sites were found which corresponded to the four binding sites found for nitrate ions in monoclinic crystals. The study suggests that most of the anion-binding sites in lysozyme remain unchanged even when different anions and different crystal forms of lysozyme are employed.
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The line below this paragraph, {{ABSTRACT_PUBMED_9757106}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 9757106 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9757106}}
==About this Structure==
==About this Structure==
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[[Category: Hydrolase]]
[[Category: Hydrolase]]
[[Category: O-glycosyl hydrolase]]
[[Category: O-glycosyl hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:52:31 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 17:54:33 2008''

Revision as of 14:54, 30 June 2008

Image:1azf.png

Template:STRUCTURE 1azf

CHICKEN EGG WHITE LYSOZYME CRYSTAL GROWN IN BROMIDE SOLUTION

Template:ABSTRACT PUBMED 9757106

About this Structure

1AZF is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.

Reference

Locations of bromide ions in tetragonal lysozyme crystals., Lim K, Nadarajah A, Forsythe EL, Pusey ML, Acta Crystallogr D Biol Crystallogr. 1998 Sep 1;54(Pt 5):899-904. PMID:9757106

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