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| | <SX load='4v91' size='340' side='right' viewer='molstar' caption='[[4v91]], [[Resolution|resolution]] 3.70Å' scene=''> | | <SX load='4v91' size='340' side='right' viewer='molstar' caption='[[4v91]], [[Resolution|resolution]] 3.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4v91]] is a 45 chain structure with sequence from [http://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4cuv 4cuv] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4cuw 4cuw]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4V91 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=4V91 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4v91]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4cuv 4cuv] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4cuw 4cuw]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4V91 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4V91 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4cux|4cux]], [[4cuy|4cuy]]</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4v91 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4v91 OCA], [https://pdbe.org/4v91 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4v91 RCSB], [https://www.ebi.ac.uk/pdbsum/4v91 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4v91 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=4v91 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4v91 OCA], [http://pdbe.org/4v91 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4v91 RCSB], [http://www.ebi.ac.uk/pdbsum/4v91 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4v91 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | [[Category: Kluyveromyces lactis]] | | [[Category: Kluyveromyces lactis]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Bai, X]] | + | [[Category: Bai X]] |
| - | [[Category: Fernandez, I S]] | + | [[Category: Fernandez IS]] |
| - | [[Category: Ramakrishnan, V]] | + | [[Category: Ramakrishnan V]] |
| - | [[Category: Scheres, S H.W]] | + | [[Category: Scheres SHW]] |
| - | [[Category: Initiation]]
| + | |
| - | [[Category: Ire]]
| + | |
| - | [[Category: Ribosome]]
| + | |
| - | [[Category: Translation]]
| + | |
| Structural highlights
Publication Abstract from PubMed
The cricket paralysis virus internal ribosome entry site (CrPV-IRES) is a folded structure in a viral mRNA that allows initiation of translation in the absence of any host initiation factors. By using recent advances in single-particle electron cryomicroscopy, we have solved the structure of CrPV-IRES bound to the ribosome of the yeast Kluyveromyces lactis in both the canonical and rotated states at overall resolutions of 3.7 and 3.8 A, respectively. In both states, the pseudoknot PKI of the CrPV-IRES mimics a tRNA/mRNA interaction in the decoding center of the A site of the 40S ribosomal subunit. The structure and accompanying factor-binding data show that CrPV-IRES binding mimics a pretranslocation rather than initiation state of the ribosome. Translocation of the IRES by elongation factor 2 (eEF2) is required to bring the first codon of the mRNA into the A site and to allow the start of translation.
Initiation of translation by cricket paralysis virus IRES requires its translocation in the ribosome.,Fernandez IS, Bai XC, Murshudov G, Scheres SH, Ramakrishnan V Cell. 2014 May 8;157(4):823-31. doi: 10.1016/j.cell.2014.04.015. Epub 2014 May 1. PMID:24792965[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Fernandez IS, Bai XC, Murshudov G, Scheres SH, Ramakrishnan V. Initiation of translation by cricket paralysis virus IRES requires its translocation in the ribosome. Cell. 2014 May 8;157(4):823-31. doi: 10.1016/j.cell.2014.04.015. Epub 2014 May 1. PMID:24792965 doi:http://dx.doi.org/10.1016/j.cell.2014.04.015
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