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| | <SX load='5a5u' size='340' side='right' viewer='molstar' caption='[[5a5u]], [[Resolution|resolution]] 9.00Å' scene=''> | | <SX load='5a5u' size='340' side='right' viewer='molstar' caption='[[5a5u]], [[Resolution|resolution]] 9.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5a5u]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A5U OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5A5U FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5a5u]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A5U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5A5U FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5a5u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a5u OCA], [https://pdbe.org/5a5u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5a5u RCSB], [https://www.ebi.ac.uk/pdbsum/5a5u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5a5u ProSAT]</span></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5a5t|5a5t]]</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5a5u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a5u OCA], [http://pdbe.org/5a5u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5a5u RCSB], [http://www.ebi.ac.uk/pdbsum/5a5u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5a5u ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/EIF3I_YEAST EIF3I_YEAST]] Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. | + | [https://www.uniprot.org/uniprot/G1SZ03_RABIT G1SZ03_RABIT] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| | [[Category: Oryctolagus cuniculus]] | | [[Category: Oryctolagus cuniculus]] |
| - | [[Category: Dhote, V]] | + | [[Category: Dhote V]] |
| - | [[Category: Frank, J]] | + | [[Category: Frank J]] |
| - | [[Category: Hashem, Y]] | + | [[Category: Hashem Y]] |
| - | [[Category: Hellen, C U.T]] | + | [[Category: Hellen CUT]] |
| - | [[Category: Kuhn, L]] | + | [[Category: Kuhn L]] |
| - | [[Category: Pestova, T V]] | + | [[Category: Pestova TV]] |
| - | [[Category: Des-Georges, A]] | + | [[Category: Des-Georges A]] |
| - | [[Category: Eif3]]
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| - | [[Category: Eif3 octamer core]]
| + | |
| - | [[Category: Eif3d]]
| + | |
| - | [[Category: Eif3g/i/b]]
| + | |
| - | [[Category: Mammalian preinitiation 34s complex]]
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| - | [[Category: Translation]]
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| Structural highlights
Function
G1SZ03_RABIT
Publication Abstract from PubMed
During eukaryotic translation initiation, 43S complexes, comprising a 40S ribosomal subunit, initiator transfer RNA and initiation factors (eIF) 2, 3, 1 and 1A, attach to the 5'-terminal region of messenger RNA and scan along it to the initiation codon. Scanning on structured mRNAs also requires the DExH-box protein DHX29. Mammalian eIF3 contains 13 subunits and participates in nearly all steps of translation initiation. Eight subunits having PCI (proteasome, COP9 signalosome, eIF3) or MPN (Mpr1, Pad1, amino-terminal) domains constitute the structural core of eIF3, to which five peripheral subunits are flexibly linked. Here we present a cryo-electron microscopy structure of eIF3 in the context of the DHX29-bound 43S complex, showing the PCI/MPN core at approximately 6 A resolution. It reveals the organization of the individual subunits and their interactions with components of the 43S complex. We were able to build near-complete polyalanine-level models of the eIF3 PCI/MPN core and of two peripheral subunits. The implications for understanding mRNA ribosomal attachment and scanning are discussed.
Structure of mammalian eIF3 in the context of the 43S preinitiation complex.,des Georges A, Dhote V, Kuhn L, Hellen CU, Pestova TV, Frank J, Hashem Y Nature. 2015 Sep 24;525(7570):491-5. doi: 10.1038/nature14891. Epub 2015 Sep 7. PMID:26344199[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ des Georges A, Dhote V, Kuhn L, Hellen CU, Pestova TV, Frank J, Hashem Y. Structure of mammalian eIF3 in the context of the 43S preinitiation complex. Nature. 2015 Sep 24;525(7570):491-5. doi: 10.1038/nature14891. Epub 2015 Sep 7. PMID:26344199 doi:http://dx.doi.org/10.1038/nature14891
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