1azh

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[[Image:1azh.jpg|left|200px]]
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{{Seed}}
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[[Image:1azh.png|left|200px]]
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{{STRUCTURE_1azh| PDB=1azh | SCENE= }}
{{STRUCTURE_1azh| PDB=1azh | SCENE= }}
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'''THREE-DIMENSIONAL STRUCTURES OF THREE ENGINEERED CELLULOSE-BINDING DOMAINS OF CELLOBIOHYDROLASE I FROM TRICHODERMA REESEI, NMR, 14 STRUCTURES'''
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===THREE-DIMENSIONAL STRUCTURES OF THREE ENGINEERED CELLULOSE-BINDING DOMAINS OF CELLOBIOHYDROLASE I FROM TRICHODERMA REESEI, NMR, 14 STRUCTURES===
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==Overview==
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Three-dimensional solution structures for three engineered, synthetic CBDs (Y5A, Y31A, and Y32A) of cellobiohydrolase I (CBHI) from Trichoderma reesei were studied with nuclear magnetic resonance (NMR) and circular dichroism (CD) spectroscopy. According to CD measurements the antiparallel beta-sheet structure of the CBD fold was preserved in all engineered peptides. The three-dimensional NMR-based structures of Y31A and Y32A revealed only small local changes due to mutations in the flat face of CBD, which is expected to bind to crystalline cellulose. Therefore, the structural roles of Y31 and Y32 are minor, but their functional importance is obvious because these mutants do not bind strongly to cellulose. In the case of Y5A, the disruption of the structural framework at the N-terminus and the complete loss of binding affinity implies that Y5 has both structural and functional significance. The number of aromatic residues and their precise spatial arrangement in the flat face of the type I CBD fold appears to be critical for specific binding. A model for the CBD binding in which the three aligned aromatic rings stack onto every other glucose ring of the cellulose polymer is discussed.
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{{ABSTRACT_PUBMED_9041630}}
==About this Structure==
==About this Structure==
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1AZH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Hypocrea_jecorina Hypocrea jecorina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AZH OCA].
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1AZH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Hypocrea_jecorina Hypocrea jecorina]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AZH OCA].
==Reference==
==Reference==
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[[Category: Nuclear magnetic resonance spectroscopy]]
[[Category: Nuclear magnetic resonance spectroscopy]]
[[Category: Protein-carbohydrate interaction]]
[[Category: Protein-carbohydrate interaction]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:52:39 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 17:54:33 2008''

Revision as of 14:54, 30 June 2008

Image:1azh.png

Template:STRUCTURE 1azh

THREE-DIMENSIONAL STRUCTURES OF THREE ENGINEERED CELLULOSE-BINDING DOMAINS OF CELLOBIOHYDROLASE I FROM TRICHODERMA REESEI, NMR, 14 STRUCTURES

Template:ABSTRACT PUBMED 9041630

About this Structure

1AZH is a Single protein structure of sequence from Hypocrea jecorina. Full experimental information is available from OCA.

Reference

Three-dimensional structures of three engineered cellulose-binding domains of cellobiohydrolase I from Trichoderma reesei., Mattinen ML, Kontteli M, Kerovuo J, Linder M, Annila A, Lindeberg G, Reinikainen T, Drakenberg T, Protein Sci. 1997 Feb;6(2):294-303. PMID:9041630

Page seeded by OCA on Mon Jun 30 17:54:33 2008

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