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| | <SX load='5m0r' size='340' side='right' viewer='molstar' caption='[[5m0r]], [[Resolution|resolution]] 8.20Å' scene=''> | | <SX load='5m0r' size='340' side='right' viewer='molstar' caption='[[5m0r]], [[Resolution|resolution]] 8.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5m0r]] is a 22 chain structure with sequence from [http://en.wikipedia.org/wiki/Mvv Mvv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M0R OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5M0R FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5m0r]] is a 22 chain structure with sequence from [https://en.wikipedia.org/wiki/Visna_lentivirus_(strain_1514) Visna lentivirus (strain 1514)], [https://en.wikipedia.org/wiki/Visna/maedi_virus_EV1_KV1772 Visna/maedi virus EV1 KV1772] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M0R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5M0R FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pol ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36374 MVV])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 8.2Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5m0r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m0r OCA], [http://pdbe.org/5m0r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5m0r RCSB], [http://www.ebi.ac.uk/pdbsum/5m0r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5m0r ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5m0r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m0r OCA], [https://pdbe.org/5m0r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5m0r RCSB], [https://www.ebi.ac.uk/pdbsum/5m0r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5m0r ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/POL_VILVK POL_VILVK]] During replicative cycle of retroviruses, the reverse-transcribed viral DNA is integrated into the host chromosome by the viral integrase enzyme. RNase H activity is associated with the reverse transcriptase. | + | [https://www.uniprot.org/uniprot/POL_VILVK POL_VILVK] During replicative cycle of retroviruses, the reverse-transcribed viral DNA is integrated into the host chromosome by the viral integrase enzyme. RNase H activity is associated with the reverse transcriptase. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </SX> | | </SX> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Mvv]] | + | [[Category: Synthetic construct]] |
| - | [[Category: Ballandras-Colas, A]] | + | [[Category: Visna/maedi virus EV1 KV1772]] |
| - | [[Category: Cherepanov, P]] | + | [[Category: Ballandras-Colas A]] |
| - | [[Category: Costa, A]] | + | [[Category: Cherepanov P]] |
| - | [[Category: Kotecha, A]] | + | [[Category: Costa A]] |
| - | [[Category: Locke, J]] | + | [[Category: Kotecha A]] |
| - | [[Category: Maskell, D]] | + | [[Category: Locke J]] |
| - | [[Category: Pye, V E]] | + | [[Category: Maskell D]] |
| - | [[Category: Dna-binding]]
| + | [[Category: Pye VE]] |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Integrase]]
| + | |
| - | [[Category: Lentivirus]]
| + | |
| - | [[Category: Retrovirus]]
| + | |
| - | [[Category: Rnaseh fold]]
| + | |
| - | [[Category: Zn-binding]]
| + | |
| Structural highlights
Function
POL_VILVK During replicative cycle of retroviruses, the reverse-transcribed viral DNA is integrated into the host chromosome by the viral integrase enzyme. RNase H activity is associated with the reverse transcriptase.
Publication Abstract from PubMed
Retroviral integrase (IN) functions within the intasome nucleoprotein complex to catalyze insertion of viral DNA into cellular chromatin. Using cryo-electron microscopy, we now visualize the functional maedi-visna lentivirus intasome at 4.9 angstrom resolution. The intasome comprises a homo-hexadecamer of IN with a tetramer-of-tetramers architecture featuring eight structurally distinct types of IN protomers supporting two catalytically competent subunits. The conserved intasomal core, previously observed in simpler retroviral systems, is formed between two IN tetramers, with a pair of C-terminal domains from flanking tetramers completing the synaptic interface. Our results explain how HIV-1 IN, which self-associates into higher-order multimers, can form a functional intasome, reconcile the bulk of early HIV-1 IN biochemical and structural data, and provide a lentiviral platform for design of HIV-1 IN inhibitors.
A supramolecular assembly mediates lentiviral DNA integration.,Ballandras-Colas A, Maskell DP, Serrao E, Locke J, Swuec P, Jonsson SR, Kotecha A, Cook NJ, Pye VE, Taylor IA, Andresdottir V, Engelman AN, Costa A, Cherepanov P Science. 2017 Jan 6;355(6320):93-95. doi: 10.1126/science.aah7002. PMID:28059770[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Ballandras-Colas A, Maskell DP, Serrao E, Locke J, Swuec P, Jonsson SR, Kotecha A, Cook NJ, Pye VE, Taylor IA, Andresdottir V, Engelman AN, Costa A, Cherepanov P. A supramolecular assembly mediates lentiviral DNA integration. Science. 2017 Jan 6;355(6320):93-95. doi: 10.1126/science.aah7002. PMID:28059770 doi:http://dx.doi.org/10.1126/science.aah7002
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