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| | <SX load='5n6w' size='340' side='right' viewer='molstar' caption='[[5n6w]], [[Resolution|resolution]] 4.20Å' scene=''> | | <SX load='5n6w' size='340' side='right' viewer='molstar' caption='[[5n6w]], [[Resolution|resolution]] 4.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5n6w]] is a 16 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N6W OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5N6W FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5n6w]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N6W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5N6W FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RS1, XLRS1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.2Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5n6w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n6w OCA], [http://pdbe.org/5n6w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n6w RCSB], [http://www.ebi.ac.uk/pdbsum/5n6w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n6w ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5n6w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n6w OCA], [https://pdbe.org/5n6w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5n6w RCSB], [https://www.ebi.ac.uk/pdbsum/5n6w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5n6w ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Disease == | | == Disease == |
| - | [[http://www.uniprot.org/uniprot/XLRS1_HUMAN XLRS1_HUMAN]] X-linked retinoschisis. The disease is caused by mutations affecting the gene represented in this entry. | + | [https://www.uniprot.org/uniprot/XLRS1_HUMAN XLRS1_HUMAN] X-linked retinoschisis. The disease is caused by mutations affecting the gene represented in this entry. |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/XLRS1_HUMAN XLRS1_HUMAN]] May be active in cell adhesion processes during retinal development. | + | [https://www.uniprot.org/uniprot/XLRS1_HUMAN XLRS1_HUMAN] May be active in cell adhesion processes during retinal development. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </SX> | | </SX> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Baldock, C]] | + | [[Category: Baldock C]] |
| - | [[Category: Collins, R F]] | + | [[Category: Collins RF]] |
| - | [[Category: Jones, R P.O]] | + | [[Category: Jones RPO]] |
| - | [[Category: Owens, T W]] | + | [[Category: Owens TW]] |
| - | [[Category: Ramsay, E P]] | + | [[Category: Ramsay EP]] |
| - | [[Category: Roseman, A]] | + | [[Category: Roseman A]] |
| - | [[Category: Siebert, C A]] | + | [[Category: Siebert CA]] |
| - | [[Category: Wang, T]] | + | [[Category: Wang T]] |
| - | [[Category: Retinoschisin discoidin domain retinal structure]]
| + | |
| - | [[Category: Structural protein]]
| + | |
| Structural highlights
Disease
XLRS1_HUMAN X-linked retinoschisis. The disease is caused by mutations affecting the gene represented in this entry.
Function
XLRS1_HUMAN May be active in cell adhesion processes during retinal development.
Publication Abstract from PubMed
Retinoschisin, an octameric retinal-specific protein, is essential for retinal architecture with mutations causing X-linked retinoschisis (XLRS), a monogenic form of macular degeneration. Most XLRS-associated mutations cause intracellular retention, however a subset are secreted as octamers and the cause of their pathology is ill-defined. Therefore, here we investigated the solution structure of the retinoschisin monomer and the impact of two XLRS-causing mutants using a combinatorial approach of biophysics and cryo-EM. The retinoschisin monomer has an elongated structure which persists in the octameric assembly. Retinoschisin forms a dimer of octamers with each octameric ring adopting a planar propeller structure. Comparison of the octamer with the hexadecamer structure indicated little conformational change in the retinoschisin octamer upon dimerization, suggesting that the octamer provides a stable interface for the construction of the hexadecamer. The H207Q XLRS-associated mutation was found in the interface between octamers and destabilized both monomeric and octameric retinoschisin. Octamer dimerization is consistent with the adhesive function of retinoschisin supporting interactions between retinal cell layers, so disassembly would prevent structural coupling between opposing membranes. In contrast, cryo-EM structural analysis of the R141H mutation at approximately 4.2A resolution was found to only cause a subtle conformational change in the propeller tips, potentially perturbing an interaction site. Together, these findings support distinct mechanisms of pathology for two classes of XLRS-associated mutations in the retinoschisin assembly.
Structural analysis of X-linked retinoschisis mutations reveals distinct classes which differentially effect retinoschisin function.,Ramsay EP, Collins RF, Owens TW, Siebert CA, Jones RP, Wang T, Roseman AM, Baldock C Hum Mol Genet. 2016 Oct 23. pii: ddw345. doi: 10.1093/hmg/ddw345. PMID:27798099[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Ramsay EP, Collins RF, Owens TW, Siebert CA, Jones RP, Wang T, Roseman AM, Baldock C. Structural analysis of X-linked retinoschisis mutations reveals distinct classes which differentially effect retinoschisin function. Hum Mol Genet. 2016 Oct 23. pii: ddw345. doi: 10.1093/hmg/ddw345. PMID:27798099 doi:http://dx.doi.org/10.1093/hmg/ddw345
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