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| | <SX load='5tsj' size='340' side='right' viewer='molstar' caption='[[5tsj]], [[Resolution|resolution]] 8.70Å' scene=''> | | <SX load='5tsj' size='340' side='right' viewer='molstar' caption='[[5tsj]], [[Resolution|resolution]] 8.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5tsj]] is a 28 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TSJ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5TSJ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5tsj]] is a 28 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TSJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5TSJ FirstGlance]. <br> |
| - | </td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/H(+)-transporting_two-sector_ATPase H(+)-transporting two-sector ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.14 3.6.3.14] </span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 8.7Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5tsj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tsj OCA], [http://pdbe.org/5tsj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5tsj RCSB], [http://www.ebi.ac.uk/pdbsum/5tsj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5tsj ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5tsj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tsj OCA], [https://pdbe.org/5tsj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5tsj RCSB], [https://www.ebi.ac.uk/pdbsum/5tsj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5tsj ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/VATF_THET8 VATF_THET8]] Produces ATP from ADP in the presence of a proton gradient across the membrane. [[http://www.uniprot.org/uniprot/VATE_THET8 VATE_THET8]] Produces ATP from ADP in the presence of a proton gradient across the membrane. [[http://www.uniprot.org/uniprot/VATC_THET8 VATC_THET8]] Produces ATP from ADP in the presence of a proton gradient across the membrane. [[http://www.uniprot.org/uniprot/VATB_THET2 VATB_THET2]] Produces ATP from ADP in the presence of a proton gradient across the membrane. The V-type beta chain is a regulatory subunit. [[http://www.uniprot.org/uniprot/VATA_THET8 VATA_THET8]] Produces ATP from ADP in the presence of a proton gradient across the membrane. The V-type alpha chain is a catalytic subunit. [[http://www.uniprot.org/uniprot/VATD_THET2 VATD_THET2]] Produces ATP from ADP in the presence of a proton gradient across the membrane. | + | [https://www.uniprot.org/uniprot/VATA_THET8 VATA_THET8] Produces ATP from ADP in the presence of a proton gradient across the membrane. The V-type alpha chain is a catalytic subunit. |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | The bacterial A/V-type ATPase/synthase rotary motor couples ATP hydrolysis/synthesis with proton translocation across biological membranes. The A/V-type ATPase/synthase from Thermus thermophilus has been extensively studied both structurally and functionally for many years. Here we provide an 8.7A resolution cryo-electron microscopy 3D reconstruction of this complex bound to single-domain antibody fragments, small monomeric antibodies containing just the variable heavy domain. Docking of known structures into the density revealed the molecular orientation of the domain antibodies, suggesting that structure determination of co-domain antibody:protein complexes could be a useful avenue for unstable or smaller proteins. Although previous studies suggested that the presence of fluoroaluminate in this complex could change the rotary state of this enzyme, we observed no gross structural rearrangements under these conditions.
| + | |
| - | | + | |
| - | Cryo-EM analysis of a domain antibody bound rotary ATPase complex.,Davies RB, Smits C, Wong AS, Stock D, Christie M, Sandin S, Stewart AG J Struct Biol. 2017 Jan 20. pii: S1047-8477(17)30013-8. doi:, 10.1016/j.jsb.2017.01.002. PMID:28115258<ref>PMID:28115258</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 5tsj" style="background-color:#fffaf0;"></div>
| + | |
| | | | |
| | ==See Also== | | ==See Also== |
| | *[[ATPase 3D structures|ATPase 3D structures]] | | *[[ATPase 3D structures|ATPase 3D structures]] |
| - | == References == | |
| - | <references/> | |
| | __TOC__ | | __TOC__ |
| | </SX> | | </SX> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Thet8]] | + | [[Category: Thermus thermophilus HB8]] |
| - | [[Category: Davies, R B]] | + | [[Category: Davies RB]] |
| - | [[Category: Sandin, S]] | + | [[Category: Sandin S]] |
| - | [[Category: Smits, C]] | + | [[Category: Smits C]] |
| - | [[Category: Stewart, A G]] | + | [[Category: Stewart AG]] |
| - | [[Category: Stock, D]] | + | [[Category: Stock D]] |
| - | [[Category: Wong, A S.W]] | + | [[Category: Wong ASW]] |
| - | [[Category: Antibody]]
| + | |
| - | [[Category: Complex]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Membrane protein]]
| + | |
| - | [[Category: V/a-atpase]]
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