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1azr

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{{STRUCTURE_1azr| PDB=1azr | SCENE= }}
{{STRUCTURE_1azr| PDB=1azr | SCENE= }}
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'''CRYSTAL STRUCTURE OF PSEUDOMONAS AERUGINOSA ZINC AZURIN MUTANT ASP47ASP AT 2.4 ANGSTROMS RESOLUTION'''
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===CRYSTAL STRUCTURE OF PSEUDOMONAS AERUGINOSA ZINC AZURIN MUTANT ASP47ASP AT 2.4 ANGSTROMS RESOLUTION===
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==Overview==
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The Pseudomonas aeruginosa azurin mutant Asn47Asp has been isolated, its spectroscopic and kinetic properties characterized, and the X-ray crystal structure of its zinc derivative determined. While the optical and electron paramagnetic resonance spectra as well as the electron-transfer activity of the mutant are very similar to the wild-type values, the Asn47Asp reduction potential is slightly increased by 20 mV. The mutant crystallized in the orthorhombic space group P2(1)2(1)2(1) with cell dimensions a = 57.8, b = 81.5 and c = 112.6 A. There are four molecules in the asymmetric unit, packed as a tetramer which consists of two independent dimers. The zinc site of this mutant structure is similar to the wild-type zinc azurin and, in particular, the metal-binding site is almost identical to the site found in the wild-type zinc-azurin structure [Nar, Huber, Messerschmidt, Filippou, Barth, Jaquinod, Kamp &amp; Canters (1992). Eur. J. Biochem. 205, 1123-1129]. The Asp47 side chain at that mutation site takes on a very similar orientation to Asn47 in the wild-type structure preserving the two hydrogen bonds with the neighbouring Thr113 NH and O(gamma)H. Therefore, the increased reduction potential of the mutant is probably a result of an altered charge distribution close to the metal site.
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{{ABSTRACT_PUBMED_15299504}}
==About this Structure==
==About this Structure==
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[[Category: Sjolin, L.]]
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[[Category: Tsai, Lc.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 17:55:53 2008''

Revision as of 14:55, 30 June 2008

Image:1azr.png

Template:STRUCTURE 1azr

CRYSTAL STRUCTURE OF PSEUDOMONAS AERUGINOSA ZINC AZURIN MUTANT ASP47ASP AT 2.4 ANGSTROMS RESOLUTION

Template:ABSTRACT PUBMED 15299504

About this Structure

1AZR is a Single protein structure of sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.

Reference

Structure of Pseudomonas aeruginosai zinc azurin mutant Asn47Asp at 2.4 A resolution., Sjolin L, Tsai LC, Langer V, Pascher T, Karlsson G, Nordling M, Nar H, Acta Crystallogr D Biol Crystallogr. 1993 Sep 1;49(Pt 5):449-57. PMID:15299504

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