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5u0a

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Current revision (15:44, 6 March 2024) (edit) (undo)
 
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<SX load='5u0a' size='340' side='right' viewer='molstar' caption='[[5u0a]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
<SX load='5u0a' size='340' side='right' viewer='molstar' caption='[[5u0a]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5u0a]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/Thefy Thefy]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U0A OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5U0A FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5u0a]] is a 14 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermobifida_fusca_YX Thermobifida fusca YX]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U0A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5U0A FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5u07|5u07]]</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Tfu_1588 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=269800 THEFY]), Tfu_1592 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=269800 THEFY]), Tfu_1590 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=269800 THEFY]), Tfu_1591 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=269800 THEFY]), Tfu_1589 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=269800 THEFY])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5u0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5u0a OCA], [https://pdbe.org/5u0a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5u0a RCSB], [https://www.ebi.ac.uk/pdbsum/5u0a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5u0a ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5u0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5u0a OCA], [http://pdbe.org/5u0a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5u0a RCSB], [http://www.ebi.ac.uk/pdbsum/5u0a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5u0a ProSAT]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q47PJ5_THEFY Q47PJ5_THEFY]
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Type I CRISPR systems feature a sequential dsDNA target searching and degradation process, by crRNA-displaying Cascade and nuclease-helicase fusion enzyme Cas3, respectively. Here we present two cryo-EM snapshots of the Thermobifida fusca type I-E Cascade: (1) unwinding 11 bp of dsDNA at the seed-sequence region to scout for sequence complementarity, and (2) further unwinding of the entire protospacer to form a full R-loop. These structures provide the much-needed temporal and spatial resolution to resolve key mechanistic steps leading to Cas3 recruitment. In the early steps, PAM recognition causes severe DNA bending, leading to spontaneous DNA unwinding to form a seed-bubble. The full R-loop formation triggers conformational changes in Cascade, licensing Cas3 to bind. The same process also generates a bulge in the non-target DNA strand, enabling its handover to Cas3 for cleavage. The combination of both negative and positive checkpoints ensures stringent yet efficient target degradation in type I CRISPR-Cas systems.
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Structure Basis for Directional R-loop Formation and Substrate Handover Mechanisms in Type I CRISPR-Cas System.,Xiao Y, Luo M, Hayes RP, Kim J, Ng S, Ding F, Liao M, Ke A Cell. 2017 Jun 29;170(1):48-60.e11. doi: 10.1016/j.cell.2017.06.012. PMID:28666122<ref>PMID:28666122</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5u0a" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
*[[CRISPR type I-E (Cascade)|CRISPR type I-E (Cascade)]]
*[[CRISPR type I-E (Cascade)|CRISPR type I-E (Cascade)]]
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== References ==
 
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<references/>
 
__TOC__
__TOC__
</SX>
</SX>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Thefy]]
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[[Category: Thermobifida fusca YX]]
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[[Category: Ding, F]]
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[[Category: Ding F]]
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[[Category: Hayes, R P]]
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[[Category: Hayes RP]]
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[[Category: Ke, A]]
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[[Category: Ke A]]
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[[Category: Kim, J]]
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[[Category: Kim J]]
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[[Category: Liao, M]]
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[[Category: Liao M]]
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[[Category: Luo, M]]
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[[Category: Luo M]]
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[[Category: Ng, S]]
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[[Category: Ng S]]
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[[Category: Xiao, Y]]
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[[Category: Xiao Y]]
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[[Category: Cascacde]]
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[[Category: Crispr-ca]]
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[[Category: Immune system]]
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[[Category: Surveillance]]
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Current revision

CRISPR RNA-guided surveillance complex

5u0a, resolution 3.30Å

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