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| | <SX load='5urx' size='340' side='right' viewer='molstar' caption='[[5urx]], [[Resolution|resolution]] 28.00Å' scene=''> | | <SX load='5urx' size='340' side='right' viewer='molstar' caption='[[5urx]], [[Resolution|resolution]] 28.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5urx]] is a 36 chain structure with sequence from [http://en.wikipedia.org/wiki/Myxococcus_xanthus Myxococcus xanthus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5URX OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5URX FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5urx]] is a 36 chain structure with sequence from [https://en.wikipedia.org/wiki/Myxococcus_xanthus_DK_1622 Myxococcus xanthus DK 1622]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5URX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5URX FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5urw|5urw]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 28Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5urx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5urx OCA], [http://pdbe.org/5urx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5urx RCSB], [http://www.ebi.ac.uk/pdbsum/5urx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5urx ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5urx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5urx OCA], [https://pdbe.org/5urx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5urx RCSB], [https://www.ebi.ac.uk/pdbsum/5urx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5urx ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/Q1D305_MYXXD Q1D305_MYXXD] |
| - | The type VI secretion system (T6SS) is a versatile molecular weapon used by many bacteria against eukaryotic hosts or prokaryotic competitors. It consists of a cytoplasmic bacteriophage tail-like structure anchored in the bacterial cell envelope via a cytoplasmic baseplate and a periplasmic membrane complex. Rapid contraction of the sheath in the bacteriophage tail-like structure propels an inner tube/spike complex through the target cell envelope to deliver effectors. While structures of purified contracted sheath and purified membrane complex have been solved, because sheaths contract upon cell lysis and purification, no structure is available for the extended sheath. Structural information about the baseplate is also lacking. Here, we use electron cryotomography to directly visualize intact T6SS structures inside Myxococcus xanthus cells. Using sub-tomogram averaging, we resolve the structure of the extended sheath and membrane-associated components including the baseplate. Moreover, we identify novel extracellular bacteriophage tail fiber-like antennae. These results provide new structural insights into how the extended sheath prevents premature disassembly and how this sophisticated machine may recognize targets.
| + | |
| - | | + | |
| - | In vivo structures of an intact type VI secretion system revealed by electron cryotomography.,Chang YW, Rettberg LA, Ortega DR, Jensen GJ EMBO Rep. 2017 May 9. pii: e201744072. doi: 10.15252/embr.201744072. PMID:28487352<ref>PMID:28487352</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 5urx" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </SX> | | </SX> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Myxococcus xanthus]] | + | [[Category: Myxococcus xanthus DK 1622]] |
| - | [[Category: Chang, Y W]] | + | [[Category: Chang Y-W]] |
| - | [[Category: Jensen, G J]] | + | [[Category: Jensen GJ]] |
| - | [[Category: Rettberg, L A]] | + | [[Category: Rettberg LA]] |
| - | [[Category: Contractile]]
| + | |
| - | [[Category: Protein machine]]
| + | |
| - | [[Category: Protein transport]]
| + | |
| - | [[Category: Secretion system]]
| + | |
| - | [[Category: Sheath]]
| + | |
| - | [[Category: T6ss]]
| + | |
| - | [[Category: Type iv secretion]]
| + | |
