6f07

From Proteopedia

(Difference between revisions)

Current revision

CBF3 Core Complex

6f07, resolution 3.60Å

Structural highlights

6f07 is a 3 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 3.6Å
Ligands:
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CBF3B_YEAST Acts as a component of the centromere DNA-binding protein complex CBF3, which is essential for chromosome segregation and movement of centromeres along microtubules. CBF3 is required for the recruitment of other kinetochore complexes to CEN DNA. It plays a role in the attachment of chromosomes to the spindle and binds selectively to a highly conserved DNA sequence called CDEIII, found in centromers and in several promoters.

Publication Abstract from PubMed

Eukaryotic chromosomes contain a specialised region known as the centromere, which forms the platform for kinetochore assembly and microtubule attachment. The centromere is distinguished by the presence of nucleosomes containing the histone H3 variant, CENP-A. In budding yeast, centromere establishment begins with the recognition of a specific DNA sequence by the CBF3 complex. This in turn facilitates CENP-A(Cse4) nucleosome deposition and kinetochore assembly. Here, we describe a 3.6 A single-particle cryo-EM reconstruction of the core CBF3 complex, incorporating the sequence-specific DNA-binding protein Cep3 together with regulatory subunits Ctf13 and Skp1. This provides the first structural data on Ctf13, defining it as an F-box protein of the leucine-rich-repeat family, and demonstrates how a novel F-box-mediated interaction between Ctf13 and Skp1 is responsible for initial assembly of the CBF3 complex.

Structural basis for assembly of the CBF3 kinetochore complex.,Leber V, Nans A, Singleton MR EMBO J. 2017 Dec 6. pii: embj.201798134. doi: 10.15252/embj.201798134. PMID:29212814^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Leber V, Nans A, Singleton MR. Structural basis for assembly of the CBF3 kinetochore complex. EMBO J. 2017 Dec 6. pii: embj.201798134. doi: 10.15252/embj.201798134. PMID:29212814 doi:http://dx.doi.org/10.15252/embj.201798134

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6f07"

Categories: Large Structures | Saccharomyces cerevisiae | Leber V | Singleton MR

@@ Line 3: / Line 3: @@
 <SX load='6f07' size='340' side='right' viewer='molstar' caption='[[6f07]], [[Resolution|resolution]] 3.60&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6f07]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6F07 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6F07 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6f07]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6F07 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6F07 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.6&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CEP3, CBF3, CBF3B, CSL1, YMR168C, YM8520.17C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), SKP1, CBF3D, YDR328C, D9798.14 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6f07 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6f07 OCA], [http://pdbe.org/6f07 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6f07 RCSB], [http://www.ebi.ac.uk/pdbsum/6f07 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6f07 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6f07 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6f07 OCA], [https://pdbe.org/6f07 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6f07 RCSB], [https://www.ebi.ac.uk/pdbsum/6f07 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6f07 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/CBF3B_YEAST CBF3B_YEAST]] Acts as a component of the centromere DNA-binding protein complex CBF3, which is essential for chromosome segregation and movement of centromeres along microtubules. CBF3 is required for the recruitment of other kinetochore complexes to CEN DNA. It plays a role in the attachment of chromosomes to the spindle and binds selectively to a highly conserved DNA sequence called CDEIII, found in centromers and in several promoters. [[http://www.uniprot.org/uniprot/SKP1_YEAST SKP1_YEAST]] Essential component of the E3 ubiquitin ligase complex SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination and subsequent proteasomal degradation of target proteins like phosphorylated SIC1. Participates in the attachment of chromosomes to the spindle. Acts as a regulatory component of the centromere DNA-binding protein complex CBF3, which is essential for chromosome segregation and movement of centromeres along microtubules. CBF3 is required for the recruitment of other kinetochore complexes to CEN DNA. It plays a role in the attachment of chromosomes to the spindle and binds selectively to a highly conserved DNA sequence called CDEIII, found in centromeres and in several promoters. The association of CBF3C with CBF3D and SGT1 is required for CBF3C activation and CBF3 assembly. SKP1/CBF3D could retrieve cyclins or cyclin-CDK-like proteins into the kinetochore thus providing cell cycle-regulated kinetochore activity. Involved in the regulation of methionine biosynthesis genes. Facilitates association of CDC53 with CDC4 and of ROY1 with YPT52.<ref>PMID:8706132</ref> <ref>PMID:8706131</ref> <ref>PMID:9346238</ref> <ref>PMID:9346239</ref> <ref>PMID:21389113</ref> <ref>PMID:9499404</ref> <ref>PMID:17517885</ref>
+[https://www.uniprot.org/uniprot/CBF3B_YEAST CBF3B_YEAST] Acts as a component of the centromere DNA-binding protein complex CBF3, which is essential for chromosome segregation and movement of centromeres along microtubules. CBF3 is required for the recruitment of other kinetochore complexes to CEN DNA. It plays a role in the attachment of chromosomes to the spindle and binds selectively to a highly conserved DNA sequence called CDEIII, found in centromers and in several promoters.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 19: / Line 19: @@
 </div>
 <div class="pdbe-citations 6f07" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Centromere protein 3D structure|Centromere protein 3D structure]]
 == References ==
 <references/>
 __TOC__
 </SX>
-[[Category: Atcc 18824]]
 [[Category: Large Structures]]
-[[Category: Leber, V]]
+[[Category: Saccharomyces cerevisiae]]
-[[Category: Singleton, M R]]
+[[Category: Leber V]]
-[[Category: Cell cycle]]
+[[Category: Singleton MR]]
-[[Category: Centromere]]
-[[Category: Dna-binding]]
-[[Category: F-box]]
-[[Category: Kinetochore]]

6f07

From Proteopedia

Current revision

CBF3 Core Complex

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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