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| | <SX load='6f95' size='340' side='right' viewer='molstar' caption='[[6f95]], [[Resolution|resolution]] 3.44Å' scene=''> | | <SX load='6f95' size='340' side='right' viewer='molstar' caption='[[6f95]], [[Resolution|resolution]] 3.44Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6f95]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6F95 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6F95 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6f95]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6F95 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6F95 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.44Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6f95 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6f95 OCA], [http://pdbe.org/6f95 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6f95 RCSB], [http://www.ebi.ac.uk/pdbsum/6f95 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6f95 ProSAT]</span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6f95 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6f95 OCA], [https://pdbe.org/6f95 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6f95 RCSB], [https://www.ebi.ac.uk/pdbsum/6f95 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6f95 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/O52947_BACIU O52947_BACIU] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </SX> | | </SX> |
| - | [[Category: Vibrio subtilis ehrenberg 1835]] | + | [[Category: Bacillus subtilis]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Lowe, J]] | + | [[Category: Lowe J]] |
| - | [[Category: Szewczak-Harris, A]] | + | [[Category: Szewczak-Harris A]] |
| - | [[Category: Bacterial cytoskeleton]]
| + | |
| - | [[Category: Cytomotive filament]]
| + | |
| - | [[Category: Plasmid segregation]]
| + | |
| - | [[Category: Viral protein]]
| + | |
| Structural highlights
Function
O52947_BACIU
Publication Abstract from PubMed
Low copy-number plasmid pLS32 of Bacillus subtilis subsp. natto contains a partitioning system that ensures segregation of plasmid copies during cell division. The partitioning locus comprises actin-like protein AlfA, adaptor protein AlfB, and the centromeric sequence parN Similar to the ParMRC partitioning system from Escherichia coli plasmid R1, AlfA filaments form actin-like double helical filaments that arrange into an antiparallel bipolar spindle, which attaches its growing ends to sister plasmids through interactions with AlfB and parN Because, compared with ParM and other actin-like proteins, AlfA is highly diverged in sequence, we determined the atomic structure of nonbundling AlfA filaments to 3.4-A resolution by cryo-EM. The structure reveals how the deletion of subdomain IIB of the canonical actin fold has been accommodated by unique longitudinal and lateral contacts, while still enabling formation of left-handed, double helical, polar and staggered filaments that are architecturally similar to ParM. Through cryo-EM reconstruction of bundling AlfA filaments, we obtained a pseudoatomic model of AlfA doublets: the assembly of two filaments. The filaments are antiparallel, as required by the segregation mechanism, and exactly antiphasic with near eightfold helical symmetry, to enable efficient doublet formation. The structure of AlfA filaments and doublets shows, in atomic detail, how deletion of an entire domain of the actin fold is compensated by changes to all interfaces so that the required properties of polymerization, nucleotide hydrolysis, and antiparallel doublet formation are retained to fulfill the system's biological raison d'etre.
Cryo-EM reconstruction of AlfA from Bacillus subtilis reveals the structure of a simplified actin-like filament at 3.4-A resolution.,Szewczak-Harris A, Lowe J Proc Natl Acad Sci U S A. 2018 Mar 27;115(13):3458-3463. doi:, 10.1073/pnas.1716424115. Epub 2018 Feb 13. PMID:29440489[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Szewczak-Harris A, Lowe J. Cryo-EM reconstruction of AlfA from Bacillus subtilis reveals the structure of a simplified actin-like filament at 3.4-A resolution. Proc Natl Acad Sci U S A. 2018 Mar 27;115(13):3458-3463. doi:, 10.1073/pnas.1716424115. Epub 2018 Feb 13. PMID:29440489 doi:http://dx.doi.org/10.1073/pnas.1716424115
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