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| | <SX load='6mgv' size='340' side='right' viewer='molstar' caption='[[6mgv]], [[Resolution|resolution]] 3.10Å' scene=''> | | <SX load='6mgv' size='340' side='right' viewer='molstar' caption='[[6mgv]], [[Resolution|resolution]] 3.10Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6mgv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MGV OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6MGV FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6mgv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MGV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6MGV FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6mgw|6mgw]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.1Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CSC1, At4g22120, F1N20.220 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6mgv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mgv OCA], [https://pdbe.org/6mgv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6mgv RCSB], [https://www.ebi.ac.uk/pdbsum/6mgv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6mgv ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6mgv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mgv OCA], [http://pdbe.org/6mgv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mgv RCSB], [http://www.ebi.ac.uk/pdbsum/6mgv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mgv ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CSC1_ARATH CSC1_ARATH]] Acts as an osmosensitive calcium-permeable cation channel. Specifically conducts cations including Ca(2+), K(+) and Na(+) in vitro. Inactivation or closure of the channel is calcium-dependent.<ref>PMID:24503647</ref> | + | [https://www.uniprot.org/uniprot/CSC1_ARATH CSC1_ARATH] Acts as an osmosensitive calcium-permeable cation channel. Specifically conducts cations including Ca(2+), K(+) and Na(+) in vitro. Inactivation or closure of the channel is calcium-dependent.<ref>PMID:24503647</ref> |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | Mechanically activated ion channels underlie touch, hearing, shear-stress sensing, and response to turgor pressure. OSCA/TMEM63s are a newly-identified family of eukaryotic mechanically activated ion channels opened by membrane tension. The structural underpinnings of OSCA/TMEM63 function are not explored. Here, we elucidate high resolution cryo-electron microscopy structures of OSCA1.2, revealing a dimeric architecture containing eleven transmembrane helices per subunit and surprising topological similarities to TMEM16 proteins. We locate the ion permeation pathway within each subunit by demonstrating that a conserved acidic residue is a determinant of channel conductance. Molecular dynamics simulations reveal membrane interactions, suggesting the role of lipids in OSCA1.2 gating. These results lay a foundation to decipher how the structural organization of OSCA/TMEM63 is suited for their roles as MA ion channels.
| + | |
| - | | + | |
| - | Cryo-EM structure of the mechanically activated ion channel OSCA1.2.,Jojoa Cruz S, Saotome K, Murthy SE, Tsui CCA, Sansom MS, Patapoutian A, Ward AB Elife. 2018 Nov 1;7. pii: 41845. doi: 10.7554/eLife.41845. PMID:30382939<ref>PMID:30382939</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 6mgv" style="background-color:#fffaf0;"></div>
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| | | | |
| | ==See Also== | | ==See Also== |
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| | __TOC__ | | __TOC__ |
| | </SX> | | </SX> |
| - | [[Category: Arath]] | + | [[Category: Arabidopsis thaliana]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Jojoa-Cruz, S]] | + | [[Category: Jojoa-Cruz S]] |
| - | [[Category: Patapoutian, A]] | + | [[Category: Patapoutian A]] |
| - | [[Category: Saotome, K]] | + | [[Category: Saotome K]] |
| - | [[Category: Ward, A B]] | + | [[Category: Ward AB]] |
| - | [[Category: Mechanically activated ion channel]]
| + | |
| - | [[Category: Membrane protein]]
| + | |
