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| | <SX load='6mzc' size='340' side='right' viewer='molstar' caption='[[6mzc]], [[Resolution|resolution]] 4.50Å' scene=''> | | <SX load='6mzc' size='340' side='right' viewer='molstar' caption='[[6mzc]], [[Resolution|resolution]] 4.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6mzc]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MZC OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6MZC FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6mzc]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MZC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6MZC FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.5Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6mzc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mzc OCA], [http://pdbe.org/6mzc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mzc RCSB], [http://www.ebi.ac.uk/pdbsum/6mzc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mzc ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6mzc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mzc OCA], [https://pdbe.org/6mzc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6mzc RCSB], [https://www.ebi.ac.uk/pdbsum/6mzc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6mzc ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Disease == | | == Disease == |
| - | [[http://www.uniprot.org/uniprot/TAF2_HUMAN TAF2_HUMAN]] Microcephaly-thin corpus callosum-intellectual disability syndrome. The disease is caused by mutations affecting the gene represented in this entry. | + | [https://www.uniprot.org/uniprot/TAF2_HUMAN TAF2_HUMAN] Microcephaly-thin corpus callosum-intellectual disability syndrome. The disease is caused by mutations affecting the gene represented in this entry. |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/TAF8_HUMAN TAF8_HUMAN]] Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. Mediates both basal and activator-dependent transcription. Plays a role in the differentiation of preadipocyte fibroblasts to adipocytes, however, does not seem to play a role in differentiation of myoblasts. Required for the integration of TAF10 in the TAF complex. May be important for survival of cells of the inner cell mass which constitute the pluripotent cell population of the early embryo (By similarity). [[http://www.uniprot.org/uniprot/TAF5_HUMAN TAF5_HUMAN]] TAFs are components of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP-free TAFII complex (TFTC). TAFs components-TIIFD are essential for mediating regulation of RNA polymerase transcription. TAF5/TAFII100 interacts strongly with the histone H4-related TAF6/TAFII80 and the histone H3-related TAF9/TAFII31, as well as a stable complex comprised of both TAF5/TAFII80 and TAF6/TAFII31. Apparently weaker interactions of TAF5/TAFII100 with TBP, TAF1/TAFII250, TAF11/TAFII28, and TAF12/TAFII20, but not TAF7/TAFII55, also have been observed. [[http://www.uniprot.org/uniprot/TAF9_HUMAN TAF9_HUMAN]] Essential for cell viability. TAF9 and TAF9B are involved in transcriptional activation as well as repression of distinct but overlapping sets of genes. May have a role in gene regulation associated with apoptosis. TAFs are components of the transcription factor IID (TFIID) complex, the TBP-free TAFII complex (TFTC), the PCAF histone acetylase complex and the STAGA transcription coactivator-HAT complex. TFIID or TFTC are essential for the regulation of RNA polymerase II-mediated transcription.<ref>PMID:15899866</ref> [[http://www.uniprot.org/uniprot/TAF12_HUMAN TAF12_HUMAN]] TAFs are components of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP-free TAFII complex (TFTC). TAFs components-TIIFD are essential for mediating regulation of RNA polymerase transcription. [[http://www.uniprot.org/uniprot/TAF4_HUMAN TAF4_HUMAN]] Makes part of TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Potentiates transcriptional activation by the AF-2S of the retinoic acid, vitamin D3 and thyroid hormone. [[http://www.uniprot.org/uniprot/TAF10_HUMAN TAF10_HUMAN]] TAFs are components of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP-free TAFII complex (TFTC). TIIFD is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. [[http://www.uniprot.org/uniprot/TAF6_HUMAN TAF6_HUMAN]] TAFs are components of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP-free TAFII complex (TFTC). TIIFD is multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. [[http://www.uniprot.org/uniprot/TAF2_HUMAN TAF2_HUMAN]] Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. It requires core promoter-specific cofactors for productive transcription stimulation. TAF2 stabilizes TFIID binding to core promoter.<ref>PMID:9418870</ref> <ref>PMID:9774672</ref> | + | [https://www.uniprot.org/uniprot/TAF2_HUMAN TAF2_HUMAN] Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. It requires core promoter-specific cofactors for productive transcription stimulation. TAF2 stabilizes TFIID binding to core promoter.<ref>PMID:9418870</ref> <ref>PMID:9774672</ref> |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | The general transcription factor IID (TFIID) is a critical component of the eukaryotic transcription preinitiation complex (PIC) and is responsible for recognizing the core promoter DNA and initiating PIC assembly. We used cryo-electron microscopy (cryo-EM), chemical crosslinking-mass spectrometry (CX-MS) and biochemical reconstitution to determine the complete molecular architecture of TFIID and define the conformational landscape of TFIID in the process of TATA-box binding protein (TBP) loading onto promoter DNA. Our structural analysis revealed five structural states of TFIID in the presence of TFIIA and promoter DNA, showing that the initial binding of TFIID to the downstream promoter positions the upstream DNA and facilitates scanning of TBP for a TATA-box and the subsequent engagement of the promoter. Our findings provide a mechanistic model for the specific loading of TBP by TFIID onto the promoter.
| + | |
| - | | + | |
| - | Structure of human TFIID and mechanism of TBP loading onto promoter DNA.,Patel AB, Louder RK, Greber BJ, Grunberg S, Luo J, Fang J, Liu Y, Ranish J, Hahn S, Nogales E Science. 2018 Nov 15. pii: science.aau8872. doi: 10.1126/science.aau8872. PMID:30442764<ref>PMID:30442764</ref>
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| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 6mzc" style="background-color:#fffaf0;"></div>
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| | | | |
| | ==See Also== | | ==See Also== |
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| | [[Category: Homo sapiens]] | | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Fang, J]] | + | [[Category: Fang J]] |
| - | [[Category: Greber, B J]] | + | [[Category: Greber BJ]] |
| - | [[Category: Grunberg, S]] | + | [[Category: Grunberg S]] |
| - | [[Category: Hahn, S]] | + | [[Category: Hahn S]] |
| - | [[Category: Liu, Y]] | + | [[Category: Liu Y]] |
| - | [[Category: Louder, R K]] | + | [[Category: Louder RK]] |
| - | [[Category: Luo, J]] | + | [[Category: Luo J]] |
| - | [[Category: Nogales, E]] | + | [[Category: Nogales E]] |
| - | [[Category: Patel, A B]] | + | [[Category: Patel AB]] |
| - | [[Category: Ranish, J]] | + | [[Category: Ranish J]] |
| - | [[Category: Dna]]
| + | |
| - | [[Category: Nuclear]]
| + | |
| - | [[Category: Transcription]]
| + | |
