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| | <SX load='6nf8' size='340' side='right' viewer='molstar' caption='[[6nf8]], [[Resolution|resolution]] 3.48Å' scene=''> | | <SX load='6nf8' size='340' side='right' viewer='molstar' caption='[[6nf8]], [[Resolution|resolution]] 3.48Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6nf8]] is a 32 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NF8 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6NF8 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6nf8]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NF8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NF8 FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.48Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3jd5|3jd5]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6nf8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nf8 OCA], [https://pdbe.org/6nf8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6nf8 RCSB], [https://www.ebi.ac.uk/pdbsum/6nf8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6nf8 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6nf8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nf8 OCA], [http://pdbe.org/6nf8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nf8 RCSB], [http://www.ebi.ac.uk/pdbsum/6nf8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nf8 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/IF3M_HUMAN IF3M_HUMAN]] IF-3 binds to the 28S ribosomal subunit and shifts the equilibrum between 55S ribosomes and their 39S and 28S subunits in favor of the free subunits, thus enhancing the availability of 28S subunits on which protein synthesis initiation begins.<ref>PMID:12095986</ref> [[http://www.uniprot.org/uniprot/RT34_BOVIN RT34_BOVIN]] Required for mitochondrial translation, plays a role in maintaining the stability of the small ribosomal subunit and the 12S rRNA that are required for mitoribosome formation.[UniProtKB:Q9JIK9] [[http://www.uniprot.org/uniprot/PTCD3_BOVIN PTCD3_BOVIN]] Mitochondrial RNA-binding protein that has a role in mitochondrial translation. | + | [https://www.uniprot.org/uniprot/RT17_BOVIN RT17_BOVIN] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </SX> | | </SX> |
| | [[Category: Bos taurus]] | | [[Category: Bos taurus]] |
| - | [[Category: Homo sapiens]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Agrawal, R]] | + | [[Category: Agrawal R]] |
| - | [[Category: Koripella, R]] | + | [[Category: Koripella R]] |
| - | [[Category: Sharma, M]] | + | [[Category: Sharma M]] |
| - | [[Category: Mitochondrial translation initiation factor 3]]
| + | |
| - | [[Category: Ribosomal protein]]
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| Structural highlights
Function
RT17_BOVIN
Publication Abstract from PubMed
The human mitochondrial translational initiation factor 3 (IF3mt) carries mitochondrial-specific amino acid extensions at both its N and C termini (N- and C-terminal extensions [NTE and CTE, respectively]), when compared with its eubacterial counterpart. Here we present 3.3- to 3.5-A-resolution cryoelectron microscopic structures of the mammalian 28S mitoribosomal subunit in complex with human IF3mt. Unique contacts observed between the 28S subunit and N-terminal domain of IF3mt explain its unusually high affinity for the 28S subunit, whereas the position of the mito-specific NTE suggests NTE's role in binding of initiator tRNA to the 28S subunit. The location of the C-terminal domain (CTD) clarifies its anti-association activity, whereas the orientation of the mito-specific CTE provides a mechanistic explanation for its role in destabilizing initiator tRNA in the absence of mRNA. Furthermore, our structure hints at a possible role of the CTD in recruiting leaderless mRNAs for translation initiation. Our findings highlight unique features of IF3mt in mitochondrial translation initiation.
Structure of Human Mitochondrial Translation Initiation Factor 3 Bound to the Small Ribosomal Subunit.,Koripella RK, Sharma MR, Haque ME, Risteff P, Spremulli LL, Agrawal RK iScience. 2019 Feb 22;12:76-86. doi: 10.1016/j.isci.2018.12.030. Epub 2019 Jan 3. PMID:30677741[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Koripella RK, Sharma MR, Haque ME, Risteff P, Spremulli LL, Agrawal RK. Structure of Human Mitochondrial Translation Initiation Factor 3 Bound to the Small Ribosomal Subunit. iScience. 2019 Feb 22;12:76-86. doi: 10.1016/j.isci.2018.12.030. Epub 2019 Jan 3. PMID:30677741 doi:http://dx.doi.org/10.1016/j.isci.2018.12.030
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