6oya

From Proteopedia

(Difference between revisions)

Current revision

Structure of the Rhodopsin-Transducin-Nanobody Complex

6oya, resolution 3.30Å

Structural highlights

6oya is a 5 chain structure with sequence from Bos taurus and Lama glama. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 3.3Å
Ligands:
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GNAT1_BOVIN Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase.

Publication Abstract from PubMed

Rhodopsin (Rho), a prototypical G-protein-coupled receptor (GPCR) in vertebrate vision, activates the G-protein transducin (GT) by catalyzing GDP-GTP exchange on its alpha subunit (GalphaT). To elucidate the determinants of GT coupling and activation, we obtained cryo-EM structures of a fully functional, light-activated Rho-GT complex in the presence and absence of a G-protein-stabilizing nanobody. The structures illustrate how GT overcomes its low basal activity by engaging activated Rho in a conformation distinct from other GPCR-G-protein complexes. Moreover, the nanobody-free structures reveal native conformations of G-protein components and capture three distinct conformers showing the GalphaT helical domain (alphaHD) contacting the Gbetagamma subunits. These findings uncover the molecular underpinnings of G-protein activation by visual rhodopsin and shed new light on the role played by Gbetagamma during receptor-catalyzed nucleotide exchange.

Structures of the Rhodopsin-Transducin Complex: Insights into G-Protein Activation.,Gao Y, Hu H, Ramachandran S, Erickson JW, Cerione RA, Skiniotis G Mol Cell. 2019 Jun 24. pii: S1097-2765(19)30440-X. doi:, 10.1016/j.molcel.2019.06.007. PMID:31300275^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Gao Y, Hu H, Ramachandran S, Erickson JW, Cerione RA, Skiniotis G. Structures of the Rhodopsin-Transducin Complex: Insights into G-Protein Activation. Mol Cell. 2019 Jun 24. pii: S1097-2765(19)30440-X. doi:, 10.1016/j.molcel.2019.06.007. PMID:31300275 doi:http://dx.doi.org/10.1016/j.molcel.2019.06.007

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6oya"

@@ Line 3: / Line 3: @@
 <SX load='6oya' size='340' side='right' viewer='molstar' caption='[[6oya]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6oya]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin], [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Camelus_glama Camelus glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OYA OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6OYA FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6oya]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OYA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6OYA FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6oya FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6oya OCA], [http://pdbe.org/6oya PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6oya RCSB], [http://www.ebi.ac.uk/pdbsum/6oya PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6oya ProSAT]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6oya FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6oya OCA], [https://pdbe.org/6oya PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6oya RCSB], [https://www.ebi.ac.uk/pdbsum/6oya PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6oya ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/GNAT1_BOVIN GNAT1_BOVIN]] Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase. [[http://www.uniprot.org/uniprot/GBB1_BOVIN GBB1_BOVIN]] Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. [[http://www.uniprot.org/uniprot/OPSD_BOVIN OPSD_BOVIN]] Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal (By similarity).<ref>PMID:16908857</ref> <ref>PMID:17060607</ref>
+[https://www.uniprot.org/uniprot/GNAT1_BOVIN GNAT1_BOVIN] Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 20: / Line 21: @@
 ==See Also==
+*[[Antibody 3D structures|Antibody 3D structures]]
 *[[Rhodopsin 3D structures|Rhodopsin 3D structures]]
 *[[Transducin 3D structures|Transducin 3D structures]]
+*[[3D structures of non-human antibody|3D structures of non-human antibody]]
 == References ==
 <references/>
@@ Line 27: / Line 30: @@
 </SX>
 [[Category: Bos taurus]]
-[[Category: Bovin]]
+[[Category: Lama glama]]
-[[Category: Camelus glama]]
 [[Category: Large Structures]]
-[[Category: Cerione, R A]]
+[[Category: Cerione RA]]
-[[Category: Erickson, J W]]
+[[Category: Erickson JW]]
-[[Category: Gao, Y]]
+[[Category: Gao Y]]
-[[Category: Hu, H]]
+[[Category: Hu H]]
-[[Category: Ramachandran, S]]
+[[Category: Ramachandran S]]
-[[Category: Skiniotis, G]]
+[[Category: Skiniotis G]]
-[[Category: Complex]]
-[[Category: G protein]]
-[[Category: Gpcr]]
-[[Category: Signaling protein-immune system complex]]

6oya

From Proteopedia

Current revision

Structure of the Rhodopsin-Transducin-Nanobody Complex

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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