6u0h

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Current revision (09:28, 20 March 2024) (edit) (undo)
 
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<SX load='6u0h' size='340' side='right' viewer='molstar' caption='[[6u0h]], [[Resolution|resolution]] 4.30&Aring;' scene=''>
<SX load='6u0h' size='340' side='right' viewer='molstar' caption='[[6u0h]], [[Resolution|resolution]] 4.30&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6u0h]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Tetth Tetth]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U0H OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6U0H FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6u0h]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Tetrahymena_thermophila Tetrahymena thermophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U0H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6U0H FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.3&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BTU1, BTU2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5911 TETTH])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6u0h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u0h OCA], [http://pdbe.org/6u0h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6u0h RCSB], [http://www.ebi.ac.uk/pdbsum/6u0h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6u0h ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6u0h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u0h OCA], [https://pdbe.org/6u0h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6u0h RCSB], [https://www.ebi.ac.uk/pdbsum/6u0h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6u0h ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/TBA_TETTH TBA_TETTH]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [[http://www.uniprot.org/uniprot/TBB_TETTH TBB_TETTH]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
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[https://www.uniprot.org/uniprot/TBA_TETTH TBA_TETTH] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Cilia, the hair-like protrusions that beat at high frequencies to propel a cell or move fluid around are composed of radially bundled doublet microtubules. In this study, we present a near-atomic resolution map of the Tetrahymena doublet microtubule by cryoelectron microscopy. The map demonstrates that the network of microtubule inner proteins weaves into the tubulin lattice and forms an inner sheath. From mass spectrometry data and de novo modeling, we identified Rib43a proteins as the filamentous microtubule inner proteins in the protofilament ribbon region. The Rib43a-tubulin interaction leads to an elongated tubulin dimer distance every 2 dimers. In addition, the tubulin lattice structure with missing microtubule inner proteins (MIPs) by sarkosyl treatment shows significant longitudinal compaction and lateral angle change between protofilaments. These results are evidence that the MIPs directly affect and stabilize the tubulin lattice. It suggests that the doublet microtubule is an intrinsically stressed filament and that this stress could be manipulated in the regulation of ciliary waveforms.
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Tubulin lattice in cilia is in a stressed form regulated by microtubule inner proteins.,Ichikawa M, Khalifa AAZ, Kubo S, Dai D, Basu K, Maghrebi MAF, Vargas J, Bui KH Proc Natl Acad Sci U S A. 2019 Oct 1;116(40):19930-19938. doi:, 10.1073/pnas.1911119116. Epub 2019 Sep 16. PMID:31527277<ref>PMID:31527277</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6u0h" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
*[[Tubulin 3D Structures|Tubulin 3D Structures]]
*[[Tubulin 3D Structures|Tubulin 3D Structures]]
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== References ==
 
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<references/>
 
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__TOC__
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[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Tetth]]
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[[Category: Tetrahymena thermophila]]
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[[Category: Basu, K]]
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[[Category: Basu K]]
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[[Category: Bui, K H]]
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[[Category: Bui KH]]
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[[Category: Ichikawa, M]]
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[[Category: Ichikawa M]]
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[[Category: Khalifa, A A.Z]]
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[[Category: Khalifa AAZ]]
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[[Category: Vargas, J]]
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[[Category: Vargas J]]
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[[Category: Axoneme]]
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[[Category: Cilia]]
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[[Category: Doublet]]
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[[Category: Microtubule inner protein]]
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[[Category: Structural protein]]
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Current revision

Tubulin lattice of the ciliary doublet microtubule from Tetrahymena thermophila

6u0h, resolution 4.30Å

Proteopedia Page Contributors and Editors (what is this?)

OCA

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