6uen

<table><tr><td colspan='2'>[[6uen]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Hrsv Hrsv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UEN OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6UEN FirstGlance]. <br>

</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">L, MZ07_64039gpL, MZ07_64040gpL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11250 HRSV])</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6uen FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uen OCA], [http://pdbe.org/6uen PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6uen RCSB], [http://www.ebi.ac.uk/pdbsum/6uen PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6uen ProSAT]</span></td></tr>

[[http://www.uniprot.org/uniprot/G8EJ12_HRSV G8EJ12_HRSV]] Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently the subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The replicase mode is dependent on intracellular protein N concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals.[PIRNR:PIRNR000830]

The respiratory syncytial virus (RSV) RNA polymerase, constituted of a 250 kDa large (L) protein and tetrameric phosphoprotein (P), catalyzes three distinct enzymatic activities - nucleotide polymerization, cap addition, and cap methylation. How RSV L and P coordinate these activities is poorly understood. Here, we present a 3.67 A cryo-EM structure of the RSV polymerase (L:P) complex. The structure reveals that the RNA dependent RNA polymerase (RdRp) and capping (Cap) domains of L interact with the oligomerization domain (POD) and C-terminal domain (PCTD) of a tetramer of P. The density of the methyltransferase (MT) domain of L and the N-terminal domain of P (PNTD) is missing. Further analysis and comparison with other RNA polymerases at different stages suggest the structure we obtained is likely to be at an elongation-compatible stage. Together, these data provide enriched insights into the interrelationship, the inhibitors, and the evolutionary implications of the RSV polymerase.

 

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== References ==

[[Category: Hrsv]]

[[Category: Cao, D]]

[[Category: Gao, Y]]

[[Category: Liang, B]]

[[Category: Viral protein-transferase complex]]