6ues

<table><tr><td colspan='2'>[[6ues]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UES OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6UES FirstGlance]. <br>

</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6ues FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ues OCA], [http://pdbe.org/6ues PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ues RCSB], [http://www.ebi.ac.uk/pdbsum/6ues PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ues ProSAT]</span></td></tr>

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== Publication Abstract from PubMed ==

Specimens below 50 kDa have generally been considered too small to be analyzed by single-particle cryo-electron microscopy (cryo-EM). The high flexibility of pure RNAs makes it difficult to obtain high-resolution structures by cryo-EM. In bacteria, riboswitches regulate sulfur metabolism through binding to the S-adenosylmethionine (SAM) ligand and offer compelling targets for new antibiotics. SAM-I, SAM-I/IV, and SAM-IV are the three most commonly found SAM riboswitches, but the structure of SAM-IV is still unknown. Here, we report the structures of apo and SAM-bound SAM-IV riboswitches (119-nt, ~40 kDa) to 3.7 A and 4.1 A resolution, respectively, using cryo-EM. The structures illustrate homologies in the ligand-binding core but distinct peripheral tertiary contacts in SAM-IV compared to SAM-I and SAM-I/IV. Our results demonstrate the feasibility of resolving small RNAs with enough detail to enable detection of their ligand-binding pockets and suggest that cryo-EM could play a role in structure-assisted drug design for RNA.

 

== References ==

[[Category: Chiu, W]]

[[Category: Das, R]]

[[Category: Kappel, K]]

[[Category: Li, S]]

[[Category: Mou, T]]

[[Category: Pintilie, G]]

[[Category: Schmid, M]]

[[Category: Su, Z]]

[[Category: Zhang, K]]

[[Category: Small rna]]