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6uge

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Current revision (09:29, 20 March 2024) (edit) (undo)
 
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<SX load='6uge' size='340' side='right' viewer='molstar' caption='[[6uge]], [[Resolution|resolution]] 3.60&Aring;' scene=''>
<SX load='6uge' size='340' side='right' viewer='molstar' caption='[[6uge]], [[Resolution|resolution]] 3.60&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6uge]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Caeel Caeel]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UGE OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6UGE FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6uge]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UGE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6UGE FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.6&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mei-1, T01G9.5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 CAEEL])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Microtubule-severing_ATPase Microtubule-severing ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.6.1.1 5.6.1.1] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6uge FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uge OCA], [https://pdbe.org/6uge PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6uge RCSB], [https://www.ebi.ac.uk/pdbsum/6uge PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6uge ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6uge FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uge OCA], [http://pdbe.org/6uge PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6uge RCSB], [http://www.ebi.ac.uk/pdbsum/6uge PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6uge ProSAT]</span></td></tr>
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</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/KTNA1_CAEEL KTNA1_CAEEL]] Catalytic subunit of a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays. Required specifically for meiotic spindle formation in the female germline; the presence of this protein is inimical to the formation of mitotic spindles (PubMed:8027178, PubMed:10809666, PubMed:12885567). In body wall muscles, regulates organization of myosin thick filaments (PubMed:22621901).[HAMAP-Rule:MF_03023]<ref>PMID:10809666</ref> <ref>PMID:12885567</ref> <ref>PMID:22621901</ref> <ref>PMID:8027178</ref>
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[https://www.uniprot.org/uniprot/KTNA1_CAEEL KTNA1_CAEEL] Catalytic subunit of a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays. Required specifically for meiotic spindle formation in the female germline; the presence of this protein is inimical to the formation of mitotic spindles (PubMed:8027178, PubMed:10809666, PubMed:12885567). In body wall muscles, regulates organization of myosin thick filaments (PubMed:22621901).[HAMAP-Rule:MF_03023]<ref>PMID:10809666</ref> <ref>PMID:12885567</ref> <ref>PMID:22621901</ref> <ref>PMID:8027178</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The AAA ATPase katanin severs microtubules. It is critical in cell division, centriole biogenesis, and neuronal morphogenesis. Its mutation causes microcephaly. The microtubule templates katanin hexamerization and activates its ATPase. The structural basis for these activities and how they lead to severing is unknown. Here, we show that beta-tubulin tails are necessary and sufficient for severing. Cryoelectron microscopy (cryo-EM) structures reveal the essential tubulin tail glutamates gripped by a double spiral of electropositive loops lining the katanin central pore. Each spiral couples allosterically to the ATPase and binds alternating, successive substrate residues, with consecutive residues coordinated by adjacent protomers. This tightly couples tail binding, hexamerization, and ATPase activation. Hexamer structures in different states suggest an ATPase-driven, ratchet-like translocation of the tubulin tail through the pore. A disordered region outside the AAA core anchors katanin to the microtubule while the AAA motor exerts the forces that extract tubulin dimers and sever the microtubule.
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Katanin Grips the beta-Tubulin Tail through an Electropositive Double Spiral to Sever Microtubules.,Zehr EA, Szyk A, Szczesna E, Roll-Mecak A Dev Cell. 2019 Nov 8. pii: S1534-5807(19)30854-8. doi:, 10.1016/j.devcel.2019.10.010. PMID:31735665<ref>PMID:31735665</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6uge" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</SX>
</SX>
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[[Category: Caeel]]
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[[Category: Caenorhabditis elegans]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Microtubule-severing ATPase]]
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[[Category: Synthetic construct]]
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[[Category: Roll-Mecak, A]]
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[[Category: Roll-Mecak A]]
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[[Category: Zehr, E A]]
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[[Category: Zehr EA]]
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[[Category: Katanin]]
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[[Category: Mei-1]]
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[[Category: Microtubule cytoskeleton]]
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[[Category: Microtubule-severing]]
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[[Category: Motor protein]]
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Current revision

Katanin hexamer in the ring conformation in complex with substrate

6uge, resolution 3.60Å

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