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| | <SX load='6v1q' size='340' side='right' viewer='molstar' caption='[[6v1q]], [[Resolution|resolution]] 3.11Å' scene=''> | | <SX load='6v1q' size='340' side='right' viewer='molstar' caption='[[6v1q]], [[Resolution|resolution]] 3.11Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6v1q]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Brachidanio_rerio Brachidanio rerio]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6V1Q OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6V1Q FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6v1q]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Danio_rerio Danio rerio]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6V1Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6V1Q FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.11Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">tpcn3, gb:eh507706, TPC3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7955 Brachidanio rerio])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6v1q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6v1q OCA], [http://pdbe.org/6v1q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6v1q RCSB], [http://www.ebi.ac.uk/pdbsum/6v1q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6v1q ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6v1q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6v1q OCA], [https://pdbe.org/6v1q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6v1q RCSB], [https://www.ebi.ac.uk/pdbsum/6v1q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6v1q ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/C4IXV6_DANRE C4IXV6_DANRE] |
| - | Voltage-gated ion channels endow membranes with excitability and the means to propagate action potentials that form the basis of all neuronal signaling. We determined the structure of a voltage-gated sodium channel, two-pore channel 3 (TPC3), which generates ultralong action potentials. TPC3 is distinguished by activation only at extreme membrane depolarization (V50 approximately +75 mV), in contrast to other TPCs and NaV channels that activate between -20 and 0 mV. We present electrophysiological evidence that TPC3 voltage activation depends only on voltage sensing domain 2 (VSD2) and that each of the three gating arginines in VSD2 reduces the activation threshold. The structure presents a chemical basis for sodium selectivity, and a constricted gate suggests a closed pore consistent with extreme voltage dependence. The structure, confirmed by our electrophysiology, illustrates the configuration of a bona fide resting state voltage sensor, observed without the need for any inhibitory ligand, and independent of any chemical or mutagenic alteration.
| + | |
| - | | + | |
| - | Resting state structure of the hyperdepolarization activated two-pore channel 3.,Dickinson MS, Myasnikov A, Eriksen J, Poweleit N, Stroud RM Proc Natl Acad Sci U S A. 2020 Jan 10. pii: 1915144117. doi:, 10.1073/pnas.1915144117. PMID:31924746<ref>PMID:31924746</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 6v1q" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </SX> | | </SX> |
| - | [[Category: Brachidanio rerio]] | + | [[Category: Danio rerio]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Dickinson, M S]] | + | [[Category: Dickinson MS]] |
| - | [[Category: Stroud, R M]] | + | [[Category: Stroud RM]] |
| - | [[Category: Membrane protein]]
| + | |
| - | [[Category: Tpc3]]
| + | |
| - | [[Category: Two-pore channel]]
| + | |
| - | [[Category: Voltag-gated ion channel]]
| + | |
