6k9c

<table><tr><td colspan='2'>[[6k9c]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Nitratiruptor_phage_nrs-1 Nitratiruptor phage nrs-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K9C OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6K9C FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6k9c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k9c OCA], [http://pdbe.org/6k9c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6k9c RCSB], [http://www.ebi.ac.uk/pdbsum/6k9c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6k9c ProSAT]</span></td></tr>

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== Publication Abstract from PubMed ==

NrS-1 is the first known phage that can infect Epsilonproteobacteria, one of the predominant primary producers in the deep-sea hydrothermal vent ecosystems. NrS-1 polymerase is a multidomain enzyme and is one key component of the phage replisome. The N-terminal Prim/Pol and HBD domains are responsible for DNA polymerization and de novo primer synthesis activities of NrS-1 polymerase. However, the structure and function of the C-terminus (CTR) of NrS-1 polymerase are poorly understood. Here, we report two crystal structures, showing that NrS-1 CTR adopts one unique hexameric ring-shaped conformation. Although the central helicase domain of NrS-1 CTR shares structural similarity with the superfamily III helicases, the folds of the Head and Tail domains are completely novel. Via mutagenesis and in vitro biochemical analysis, we identified many residues important for the helicase and polymerization activities of NrS-1 polymerase. In addition to NrS-1 polymerase, our study may also help us identify and understand the functions of multidomain polymerases expressed by many NrS-1 related phages.

 

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== References ==

[[Category: Nitratiruptor phage nrs-1]]

[[Category: Chen, X]]

[[Category: Gan, J]]

[[Category: Transferase]]