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5e1r

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Revision as of 06:10, 5 July 2023

Crystal structure of pecan (carya illinoinensis) vicilin, a new food allergen

Structural highlights

5e1r is a 6 chain structure with sequence from Carya illinoinensis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.651Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

VCL_CARIL Seed storage protein.^[1] ^[2]

Publication Abstract from PubMed

The 7S vicilin and 11S legumin seed storage globulins belong to the cupin protein superfamily and are major food allergens in many foods from the "big eight" food allergen groups. Here, for the first time, pecan vicilin was found to be a food allergen. Western blot experiments revealed that 30% of 27 sera used in this study and 24% of the sera from 25 patients with double-blind, placebo controlled clinical pecan allergy contained IgE antibodies specific to pecan vicilin. This allergen consists of a low-complexity region at its N-terminal and a structured domain at the C-terminal that contains two cupin motifs and forms homotrimers. The crystal structure of recombinant pecan vicilin was determined. The refined structure gave R/Rfree values of 0.218/0.262 for all data to 2.65 A. There were two trimeric biological units in the crystallographic asymmetric unit. Pecan vicilin is also a copper protein. These data may facilitate the understanding of the nutritional value and the allergenicity relevance of the copper binding property of seed storage proteins in tree nuts.

Identification and Characterization of a New Pecan [Carya illinoinensis (Wangenh.) K. Koch] Allergen, Car i 2.,Zhang Y, Lee B, Du WX, Lyu SC, Nadeau KC, Grauke LJ, Zhang Y, Wang S, Fan Y, Yi J, McHugh TH J Agric Food Chem. 2016 May 25;64(20):4146-51. doi: 10.1021/acs.jafc.6b00884., Epub 2016 May 10. PMID:27128197^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Lee B, Zhang R, Du WX, Grauke LJ, McHugh TH, Zhang YZ. Expression, purification and crystallization of pecan (Carya illinoinensis) vicilin. Acta Crystallogr F Struct Biol Commun. 2014 Aug;70(Pt 8):1049-52. PMID:25084379 doi:10.1107/S2053230X14012369
↑ Zhang Y, Lee B, Du WX, Lyu SC, Nadeau KC, Grauke LJ, Zhang Y, Wang S, Fan Y, Yi J, McHugh TH. Identification and Characterization of a New Pecan [Carya illinoinensis (Wangenh.) K. Koch] Allergen, Car i 2. J Agric Food Chem. 2016 May 25;64(20):4146-51. doi: 10.1021/acs.jafc.6b00884., Epub 2016 May 10. PMID:27128197 doi:http://dx.doi.org/10.1021/acs.jafc.6b00884
↑ Zhang Y, Lee B, Du WX, Lyu SC, Nadeau KC, Grauke LJ, Zhang Y, Wang S, Fan Y, Yi J, McHugh TH. Identification and Characterization of a New Pecan [Carya illinoinensis (Wangenh.) K. Koch] Allergen, Car i 2. J Agric Food Chem. 2016 May 25;64(20):4146-51. doi: 10.1021/acs.jafc.6b00884., Epub 2016 May 10. PMID:27128197 doi:http://dx.doi.org/10.1021/acs.jafc.6b00884

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5e1r"

Categories: Carya illinoinensis | Large Structures | Zhang YZ

@@ Line 3: / Line 3: @@
 <StructureSection load='5e1r' size='340' side='right'caption='[[5e1r]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5e1r]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Caril Caril]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5E1R OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5E1R FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5e1r]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Carya_illinoinensis Carya illinoinensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5E1R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5E1R FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.651&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pec2a1a, pec3a1a, pec4a1a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=32201 CARIL])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5e1r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5e1r OCA], [http://pdbe.org/5e1r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5e1r RCSB], [http://www.ebi.ac.uk/pdbsum/5e1r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5e1r ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5e1r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5e1r OCA], [https://pdbe.org/5e1r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5e1r RCSB], [https://www.ebi.ac.uk/pdbsum/5e1r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5e1r ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/VCL_CARIL VCL_CARIL] Seed storage protein.<ref>PMID:25084379</ref> <ref>PMID:27128197</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Caril]]
+[[Category: Carya illinoinensis]]
 [[Category: Large Structures]]
-[[Category: Zhang, Y Z]]
+[[Category: Zhang YZ]]
-[[Category: Allergen]]
-[[Category: Food allergy]]
-[[Category: Vicilin]]

5e1r

From Proteopedia

Revision as of 06:10, 5 July 2023

Crystal structure of pecan (carya illinoinensis) vicilin, a new food allergen

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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