6oy8

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==Crystal structure of Y99G mutant of human macrophage migration inhibitory factor==
==Crystal structure of Y99G mutant of human macrophage migration inhibitory factor==
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<StructureSection load='6oy8' size='340' side='right'caption='[[6oy8]]' scene=''>
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<StructureSection load='6oy8' size='340' side='right'caption='[[6oy8]], [[Resolution|resolution]] 1.53&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OY8 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6OY8 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6oy8]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OY8 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6OY8 FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6oy8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6oy8 OCA], [http://pdbe.org/6oy8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6oy8 RCSB], [http://www.ebi.ac.uk/pdbsum/6oy8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6oy8 ProSAT]</span></td></tr>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MIF, GLIF, MMIF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6oy8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6oy8 OCA], [http://pdbe.org/6oy8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6oy8 RCSB], [http://www.ebi.ac.uk/pdbsum/6oy8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6oy8 ProSAT]</span></td></tr>
</table>
</table>
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== Disease ==
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[[http://www.uniprot.org/uniprot/MIF_HUMAN MIF_HUMAN]] Genetic variations in MIF are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:[http://omim.org/entry/604302 604302]]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
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== Function ==
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[[http://www.uniprot.org/uniprot/MIF_HUMAN MIF_HUMAN]] Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.<ref>PMID:15908412</ref> <ref>PMID:17443469</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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In proteins with multiple functions, such as macrophage migration inhibitory factor (MIF), the study of its intramolecular dynamic network can offer a unique opportunity to understand how a single protein is able to carry out several nonoverlapping functions. A dynamic mechanism that controls the MIF-induced activation of CD74 was recently discovered. In this study, the regulation of tautomerase activity was explored. The catalytic base Pro1 is found to form dynamic communications with the same allosteric node that regulates CD74 activation. Signal transmission between the allosteric and catalytic sites take place through intramolecular aromatic interactions and a hydrogen bond network that involves residues and water molecules of the MIF solvent channel. Once thought to be a consequence of trimerization, a regulatory function for the solvent channel is now defined. These results provide mechanistic insights into the regulation of catalytic activity and the role of solvent channel water molecules in MIF catalysis.
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Regulation of MIF Enzymatic Activity by an Allosteric Site at the Central Solvent Channel.,Pantouris G, Khurana L, Ma A, Skeens E, Reiss K, Batista VS, Lisi GP, Lolis EJ Cell Chem Biol. 2020 Jun 18;27(6):740-750.e5. doi:, 10.1016/j.chembiol.2020.05.001. Epub 2020 May 19. PMID:32433911<ref>PMID:32433911</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6oy8" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Human]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Lolis E]]
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[[Category: Lolis, E]]
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[[Category: Pantouris G]]
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[[Category: Pantouris, G]]
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[[Category: Isomerase]]
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[[Category: Mutation]]
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[[Category: Trimeric]]

Revision as of 11:22, 22 July 2020

Crystal structure of Y99G mutant of human macrophage migration inhibitory factor

PDB ID 6oy8

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